Assimilation of metal ions bound to porphyrins or porphyrin-peptides by Vibrio vulnificus, a human pathogen inhabiting estuarine and marine environments

Shin-ichi Miyoshi, Tomoko Sasaki, Nahoko Kaku, Takaharu Inoue, Natsuki Uozumi, Yoko Maehara, Hiroshi Nakao

Research output: Contribution to journalArticle

Abstract

Vibrio vulnificus, a ubiquitous microorganism in aquatic environments, causes serious septicemia to the immunocompromised host. In addition to protoheme, this species can utilize FeTCPP [ferric tetrakis (4-carboxyphenyl) porphine] as an iron source. In the present study, heme c bound covalently to the protein in cytochrome c, as well as the Fe-TCPP complex formed with a nanopeptide with a high affinity, was found to be useful iron sources for V. vulnificus. This bacterium was also revealed to use Zn-TCPP as a single zinc source. However, other metalloporphyrins such as Mn-TCPP and Pt-TCPP delayed the bacterial growth in the broth containing Fe-TCPP, suggesting interference in the iron assimilation. These results indicate that V. vulnificus may acquire metal ions from both free and peptide-bound metalloporphyrins.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiocontrol Science
Volume15
Issue number1
Publication statusPublished - 2010

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Keywords

  • Heme
  • Iron source
  • Metalloporphyrin
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

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