Assessment of the bioartificial liver support system that consisted of encapsulated multicellular spheroids of rat hepatocyte and a hollow fiber cartridge

H. Shiraha, N. Koide, T. Shinji, S. Nakaji, N. Hayashi, T. Tsuji

Research output: Contribution to journalArticle

Abstract

We designed the hollow fiber type bio-artificial liver support system in which encapsulated multicellular spheroids were utilized as a bioreactor. Multicellular spheroids of rat hepatocyte were encapsulated into microdroplets of agarose. The microdroplets containing 9x107 rat hepatocyte were packed into the extra capillary space of a hollow fiber cartridge. Hormonal defined medium, 150ml including reservoir volume, was circulated in a closed circuit in which the cartridge was inserted. The levels of dissolved oxygen and pH were monitored and were automatically regulated to maintain at a constant range for 7Z hours. Albumin was accumulated in the circuit at a rate of 2.0 mg/l/hour and transferrin was at a rate of 0.84 mg/l/hour until 24 hours. The spheroids were 86% viable after the circulating culture period. These results indicate that the system described in this paper is one of useful prototype modules to develop a bio-artificial liver support.

Original languageEnglish
Pages (from-to)740-743
Number of pages4
JournalJapanese Journal of Artificial Organs
Volume24
Issue number3
Publication statusPublished - Jan 1 1995

Keywords

  • artificial liver support system
  • encapsulation
  • hollow fiber
  • multicellular spheroid

ASJC Scopus subject areas

  • Biophysics

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