Assessment of peripheral tissue perfusion disorder in streptozotocin-induced diabetic rats using dynamic contrast-enhanced MRI

Yuto Kashiwagi, Mika Nodaira, Misato Amitani, Kenya Murase, Koji Abe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: To assess peripheral tissue perfusion disorder in streptozotocin (STZ)-induced diabetic rats by using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Materials and Methods: A rat diabetes model was produced by intravenous injection of STZ. Diabetic rats were sustainably treated with either saline or insulin using an Alzet osmotic pump. Hind paw tissue perfusion was measured by signal intensity (SI) enhancement after gadolinium diethylenetriaminepentaacetic acid injection in DCE-MRI study and quantified using the initial area under the SI-time curve (IAUC). Peripheral tissue uptake of [ 14C]iodoantipyrine (IAP) was also determined as a marker of tissue blood flow for comparison with the IAUC value indicating tissue perfusion. Results: STZ caused hyperglycemia at 1 and 2 weeks after injection. Treatment with insulin significantly alleviated hyperglycemia. At 2 weeks after STZ injection, peripheral tissue perfusion was clearly reduced in the diabetic rats and its reduction was significantly improved in the insulin-treated diabetic rats. Tissue perfusion evaluated by DCE-MRI was similar to the tissue blood flow measured by [ 14C]IAP uptake. Conclusion: Our findings demonstrated that DCE-MRI can assess peripheral tissue perfusion disorder in diabetes. DCE-MRI could be suitable for noninvasive evaluation of peripheral tissue perfusion in both preclinical and clinical studies. It may also be useful for developing novel drugs to protect against diabetic vascular complications.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalMagnetic Resonance Imaging
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

Fingerprint

Streptozocin
Magnetic resonance imaging
Rats
Perfusion
Tissue
Magnetic resonance
Magnetic Resonance Imaging
Insulin
Imaging techniques
Medical problems
Hyperglycemia
Injections
Blood
Diabetic Angiopathies
Gadolinium
Intravenous Injections
Pumps
Acids

Keywords

  • Dynamic contrast-enhanced MRI
  • Peripheral tissue perfusion
  • Streptozotocin-induced diabetic rat

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Biomedical Engineering

Cite this

Assessment of peripheral tissue perfusion disorder in streptozotocin-induced diabetic rats using dynamic contrast-enhanced MRI. / Kashiwagi, Yuto; Nodaira, Mika; Amitani, Misato; Murase, Kenya; Abe, Koji.

In: Magnetic Resonance Imaging, Vol. 30, No. 2, 02.2012, p. 254-260.

Research output: Contribution to journalArticle

Kashiwagi, Yuto ; Nodaira, Mika ; Amitani, Misato ; Murase, Kenya ; Abe, Koji. / Assessment of peripheral tissue perfusion disorder in streptozotocin-induced diabetic rats using dynamic contrast-enhanced MRI. In: Magnetic Resonance Imaging. 2012 ; Vol. 30, No. 2. pp. 254-260.
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