Assessment of current adhesives in class I cavity: Nondestructive imaging using optical coherence tomography and microtensile bond strength

Patricia Makishi, Suppason Thitthaweerat, Alireza Sadr, Yasushi Shimada, Adriano Luis Martins, Junji Tagami, Marcelo Giannini

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Abstract Objectives To evaluate the sealing ability and the microtensile bond strength (MTBS) of different adhesive systems bonded to dentin in class I cavities. Methods Round tapered dentin cavities (3-mm diameter, 1.5-mm height) prepared in extracted human molars were restored using composite resin (Clearfil Majesty Posterior) with two-step etch-and-rinse adhesive system (Adper Single Bond 2: ASB2), two-step self-etch adhesive (Clearfil SE Bond: CSEB), all-in-one adhesives (G-Bond Plus: GBP; Tri-S Bond Plus: TSBP), or no adhesive (Control), or bonded using low-shrinkage composite with its proper adhesive (Filtek Silorane, Silorane Adhesive System: FSS). After 24-h water storage or 10,000 cycles of thermal stress, the specimens were immersed into a contrast agent. Two and three-dimensional images were obtained using optical coherence tomography (OCT). The mean percentage of high brightness (HB%) at the interfacial zone in cross-sectional images was calculated as an indicator of contrast agent or gap at the interface. The specimens were then sectioned into beams and the MTBS measured. Results The HB% (ASB2 = TSBP = CSEB < FSS = GBP) and MTBS (CSEB = ASB2, CSEB > TSBP = GBP = FSS, ASB2 > FSS) differed significantly among the adhesives. After aging, HB% increased for GBP and FSS specimens, and the MTBS decreased for FSS specimens (ANOVA, Tukey's post hoc, p < 0.05). The HB% and MTBS were significantly and negatively correlated (p = 0.002). Confocal laser scanning and scanning electron micrographs confirmed contrast agent infiltration within the gap. Significance There was a significant correlation between sealing performance and bond strength of the adhesives in the whole cavity. After aging, the two-step systems showed equal or superior performance to the all-in-one and Silorane systems.

Original languageEnglish
Article number2584
Pages (from-to)e190-e200
JournalDental Materials
Issue number9
Publication statusPublished - Sept 1 2015
Externally publishedYes


  • Adhesive
  • Bond strength
  • Contrast agent
  • Marginal adaptation
  • Optical coherence tomography
  • Silver staining

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials


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