Assessment of chromosome 19 for genetic association in sever chronic periodontitis

Koichi Tabata, Yasuko Shimada, Hideaki Tai, Yulchi Ishihara, Toshihide Noguchi, Yoshihiko Soga, Shogo Takashiba, Genki Suzuki, Terukazu Kobayashi, Akira Oka, Tetsuo Kobayashi, Kazuhisa Yamazaki, Hidetoshi Inoko, Hiromasa Yoshio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: A genome-association study is a powerful tool for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population. Methods: We adopted a DMA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole-genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case-control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs). Results: The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (P= 0.0012 for 2x2; P

Original languageEnglish
Pages (from-to)663-671
Number of pages9
JournalJournal of Periodontology
Volume80
Issue number4
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Chromosomes, Human, Pair 19
Chronic Periodontitis
Microsatellite Repeats
Costs and Cost Analysis
Genome
DNA
Single Nucleotide Polymorphism
Healthy Volunteers
Alleles
Population
Genes

Keywords

  • Association study
  • Chronic periodontitis
  • DNA
  • Microsatellite marker
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Periodontics

Cite this

Tabata, K., Shimada, Y., Tai, H., Ishihara, Y., Noguchi, T., Soga, Y., ... Yoshio, H. (2009). Assessment of chromosome 19 for genetic association in sever chronic periodontitis. Journal of Periodontology, 80(4), 663-671. https://doi.org/10.1902/jop.2009.080516

Assessment of chromosome 19 for genetic association in sever chronic periodontitis. / Tabata, Koichi; Shimada, Yasuko; Tai, Hideaki; Ishihara, Yulchi; Noguchi, Toshihide; Soga, Yoshihiko; Takashiba, Shogo; Suzuki, Genki; Kobayashi, Terukazu; Oka, Akira; Kobayashi, Tetsuo; Yamazaki, Kazuhisa; Inoko, Hidetoshi; Yoshio, Hiromasa.

In: Journal of Periodontology, Vol. 80, No. 4, 04.2009, p. 663-671.

Research output: Contribution to journalArticle

Tabata, K, Shimada, Y, Tai, H, Ishihara, Y, Noguchi, T, Soga, Y, Takashiba, S, Suzuki, G, Kobayashi, T, Oka, A, Kobayashi, T, Yamazaki, K, Inoko, H & Yoshio, H 2009, 'Assessment of chromosome 19 for genetic association in sever chronic periodontitis', Journal of Periodontology, vol. 80, no. 4, pp. 663-671. https://doi.org/10.1902/jop.2009.080516
Tabata, Koichi ; Shimada, Yasuko ; Tai, Hideaki ; Ishihara, Yulchi ; Noguchi, Toshihide ; Soga, Yoshihiko ; Takashiba, Shogo ; Suzuki, Genki ; Kobayashi, Terukazu ; Oka, Akira ; Kobayashi, Tetsuo ; Yamazaki, Kazuhisa ; Inoko, Hidetoshi ; Yoshio, Hiromasa. / Assessment of chromosome 19 for genetic association in sever chronic periodontitis. In: Journal of Periodontology. 2009 ; Vol. 80, No. 4. pp. 663-671.
@article{2cde5faaf7f54c328e6ae55a2ba15513,
title = "Assessment of chromosome 19 for genetic association in sever chronic periodontitis",
abstract = "Background: A genome-association study is a powerful tool for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population. Methods: We adopted a DMA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole-genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case-control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs). Results: The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (P= 0.0012 for 2x2; P",
keywords = "Association study, Chronic periodontitis, DNA, Microsatellite marker, Single nucleotide polymorphism",
author = "Koichi Tabata and Yasuko Shimada and Hideaki Tai and Yulchi Ishihara and Toshihide Noguchi and Yoshihiko Soga and Shogo Takashiba and Genki Suzuki and Terukazu Kobayashi and Akira Oka and Tetsuo Kobayashi and Kazuhisa Yamazaki and Hidetoshi Inoko and Hiromasa Yoshio",
year = "2009",
month = "4",
doi = "10.1902/jop.2009.080516",
language = "English",
volume = "80",
pages = "663--671",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "4",

}

TY - JOUR

T1 - Assessment of chromosome 19 for genetic association in sever chronic periodontitis

AU - Tabata, Koichi

AU - Shimada, Yasuko

AU - Tai, Hideaki

AU - Ishihara, Yulchi

AU - Noguchi, Toshihide

AU - Soga, Yoshihiko

AU - Takashiba, Shogo

AU - Suzuki, Genki

AU - Kobayashi, Terukazu

AU - Oka, Akira

AU - Kobayashi, Tetsuo

AU - Yamazaki, Kazuhisa

AU - Inoko, Hidetoshi

AU - Yoshio, Hiromasa

PY - 2009/4

Y1 - 2009/4

N2 - Background: A genome-association study is a powerful tool for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population. Methods: We adopted a DMA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole-genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case-control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs). Results: The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (P= 0.0012 for 2x2; P

AB - Background: A genome-association study is a powerful tool for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population. Methods: We adopted a DMA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole-genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case-control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs). Results: The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (P= 0.0012 for 2x2; P

KW - Association study

KW - Chronic periodontitis

KW - DNA

KW - Microsatellite marker

KW - Single nucleotide polymorphism

UR - http://www.scopus.com/inward/record.url?scp=63849133297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63849133297&partnerID=8YFLogxK

U2 - 10.1902/jop.2009.080516

DO - 10.1902/jop.2009.080516

M3 - Article

VL - 80

SP - 663

EP - 671

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 4

ER -