Assessment of chromosome 19 for genetic association in sever chronic periodontitis

Koichi Tabata, Yasuko Shimada, Hideaki Tai, Yulchi Ishihara, Toshihide Noguchi, Yoshihito Soga, Shogo Takashiba, Genki Suzuki, Terukazu Kobayashi, Akira Oka, Tetsuo Kobayashi, Kazuhisa Yamazaki, Hidetoshi Inoko, Hiromasa Yoshio

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3 Citations (Scopus)

Abstract

Background: A genome-association study is a powerful tool for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population. Methods: We adopted a DMA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole-genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case-control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs). Results: The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (P= 0.0012 for 2x2; P<0.046 for 2 x m, where m refers to the number of polymorphic alleles observed in a population). No other SNP or MS polymorphism hypothesized to affect biologic functions in the critical region was found in the linkage disequilibrium block analysis. Conclusions: We efficiently isolated the susceptible locus for severe CP in chromosome 19 and identified a useful marker to evaluate the risk for disease. This approach can be applied to a whole-genome study in severe CP.

Original languageEnglish
Pages (from-to)663-671
Number of pages9
JournalJournal of periodontology
Volume80
Issue number4
DOIs
Publication statusPublished - Apr 2009

Keywords

  • Association study
  • Chronic periodontitis
  • DNA
  • Microsatellite marker
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Periodontics

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  • Cite this

    Tabata, K., Shimada, Y., Tai, H., Ishihara, Y., Noguchi, T., Soga, Y., Takashiba, S., Suzuki, G., Kobayashi, T., Oka, A., Kobayashi, T., Yamazaki, K., Inoko, H., & Yoshio, H. (2009). Assessment of chromosome 19 for genetic association in sever chronic periodontitis. Journal of periodontology, 80(4), 663-671. https://doi.org/10.1902/jop.2009.080516