Abstract
A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 μm3/μm2 and average thickness at 44.4 μm. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems.
| Original language | English |
|---|---|
| Pages (from-to) | 326-331 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 115 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2013 |
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Keywords
- Biofilm architecture
- Confocal laser scanning microscopy
- Flow system
- Multichannel microdevice
- Pseudomonas aeruginosa
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system. / Sanchez, Zoe; Tani, Akio; Suzuki, Nobuhiro; Kariyama, Reiko; Kumon, Hiromi; Kimbara, Kazuhide.
In: Journal of Bioscience and Bioengineering, Vol. 115, No. 3, 03.2013, p. 326-331.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system
AU - Sanchez, Zoe
AU - Tani, Akio
AU - Suzuki, Nobuhiro
AU - Kariyama, Reiko
AU - Kumon, Hiromi
AU - Kimbara, Kazuhide
PY - 2013/3
Y1 - 2013/3
N2 - A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 μm3/μm2 and average thickness at 44.4 μm. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems.
AB - A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 μm3/μm2 and average thickness at 44.4 μm. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems.
KW - Biofilm architecture
KW - Confocal laser scanning microscopy
KW - Flow system
KW - Multichannel microdevice
KW - Pseudomonas aeruginosa
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UR - http://www.scopus.com/inward/citedby.url?scp=84872331527&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2012.09.018
DO - 10.1016/j.jbiosc.2012.09.018
M3 - Article
C2 - 23085416
AN - SCOPUS:84872331527
VL - 115
SP - 326
EP - 331
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -