Abstract
Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension of Streptococcus mutans was applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel (p<0.05). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization.
| Original language | English |
|---|---|
| Article number | 014504 |
| Journal | Journal of Medical Imaging |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2016 |
| Externally published | Yes |
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Keywords
- Biofilm
- Cervical tooth
- Demineralization
- Streptococcus mutans
- Swept-source optical coherence tomography
ASJC Scopus subject areas
- Bioengineering
- Radiology Nuclear Medicine and imaging
Cite this
Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography. / Tezuka, Hiroki; Shimada, Yasushi; Matin, Khairul; Ikeda, Masaomi; Sadr, Alireza; Sumi, Yasunori; Tagami, Junji.
In: Journal of Medical Imaging, Vol. 3, No. 1, 014504, 01.01.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography
AU - Tezuka, Hiroki
AU - Shimada, Yasushi
AU - Matin, Khairul
AU - Ikeda, Masaomi
AU - Sadr, Alireza
AU - Sumi, Yasunori
AU - Tagami, Junji
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension of Streptococcus mutans was applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel (p<0.05). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization.
AB - Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension of Streptococcus mutans was applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel (p<0.05). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization.
KW - Biofilm
KW - Cervical tooth
KW - Demineralization
KW - Streptococcus mutans
KW - Swept-source optical coherence tomography
UR - http://www.scopus.com/inward/record.url?scp=85001167395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85001167395&partnerID=8YFLogxK
U2 - 10.1117/1.JMI.3.1.014504
DO - 10.1117/1.JMI.3.1.014504
M3 - Article
AN - SCOPUS:85001167395
VL - 3
JO - Journal of Medical Imaging
JF - Journal of Medical Imaging
SN - 0720-048X
IS - 1
M1 - 014504
ER -