Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography

Hiroki Tezuka, Yasushi Shimada, Khairul Matin, Masaomi Ikeda, Alireza Sadr, Yasunori Sumi, Junji Tagami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension of Streptococcus mutans was applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel (p<0.05). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization.

Original languageEnglish
Article number014504
JournalJournal of Medical Imaging
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

Fingerprint

Streptococcus mutans
Optical tomography
Optical Coherence Tomography
Biofilms
Enamels
Dental Enamel
Tooth Cervix
Dentin
Area Under Curve
Gingival Recession
Suspensions
Tooth
Monitoring

Keywords

  • Biofilm
  • Cervical tooth
  • Demineralization
  • Streptococcus mutans
  • Swept-source optical coherence tomography

ASJC Scopus subject areas

  • Bioengineering
  • Radiology Nuclear Medicine and imaging

Cite this

Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography. / Tezuka, Hiroki; Shimada, Yasushi; Matin, Khairul; Ikeda, Masaomi; Sadr, Alireza; Sumi, Yasunori; Tagami, Junji.

In: Journal of Medical Imaging, Vol. 3, No. 1, 014504, 01.01.2016.

Research output: Contribution to journalArticle

Tezuka, Hiroki ; Shimada, Yasushi ; Matin, Khairul ; Ikeda, Masaomi ; Sadr, Alireza ; Sumi, Yasunori ; Tagami, Junji. / Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography. In: Journal of Medical Imaging. 2016 ; Vol. 3, No. 1.
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