Assessment of between-instrument variations in a HPLC method for serum lipoproteins and its traceability to reference methods for total cholesterol and HDL-cholesterol

Shinichi Usui, Masakazu Nakamura, Kazuhiro Jitsukata, Masayuki Nara, Seijin Hosaki, Mitsuyo Okazaki

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background: The main purpose of this study was to evaluate the between- instrument variation of the HPLC method for the measurement of total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), VLDL- cholesterol (VLDL-C), chylomicron cholesterol (CM-C), LDL size, and HDL size. Furthermore, the accuracy of the HPLC was assessed for the determination of TC and HDL-C, compared with CDC reference methods. Methods: We used four HPLC instruments with different column-load numbers from 250 to 5000. For accuracy assessment of TC and HDL-C, we used the reference methods recommended by the CDC. Results: The values measured by the four instruments were highly correlated with each other (mean r = 0.965), and the absolute mean differences were 4-43 mg/L for TC, 4-30 mg/L for HDL-C, 0-48 mg/L for LDL-C, 7-66 mg/L for VLDL-C, 0-7 mg/L for CM-C, 0.1-0.3 nm for LDL size, and 0-0.1 nm for HDL size. For TC, the HPLC instruments showed high correlation and good agreement with the reference method: r = 0.997; total error

Original languageEnglish
Pages (from-to)63-72
Number of pages10
JournalClinical Chemistry
Volume46
Issue number1
Publication statusPublished - 2000
Externally publishedYes

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Cholesterol
HDL Cholesterol
Lipoproteins
High Pressure Liquid Chromatography
LDL Cholesterol
Chylomicrons
VLDL Cholesterol
Serum
Centers for Disease Control and Prevention (U.S.)

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Assessment of between-instrument variations in a HPLC method for serum lipoproteins and its traceability to reference methods for total cholesterol and HDL-cholesterol. / Usui, Shinichi; Nakamura, Masakazu; Jitsukata, Kazuhiro; Nara, Masayuki; Hosaki, Seijin; Okazaki, Mitsuyo.

In: Clinical Chemistry, Vol. 46, No. 1, 2000, p. 63-72.

Research output: Contribution to journalArticle

Usui, Shinichi ; Nakamura, Masakazu ; Jitsukata, Kazuhiro ; Nara, Masayuki ; Hosaki, Seijin ; Okazaki, Mitsuyo. / Assessment of between-instrument variations in a HPLC method for serum lipoproteins and its traceability to reference methods for total cholesterol and HDL-cholesterol. In: Clinical Chemistry. 2000 ; Vol. 46, No. 1. pp. 63-72.
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AU - Nakamura, Masakazu

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AU - Nara, Masayuki

AU - Hosaki, Seijin

AU - Okazaki, Mitsuyo

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