Assessment of bacterial demineralization around composite restorations using swept-source optical coherence tomography (SS-OCT)

Yuan Zhou, Yasushi Shimada, Khairul Matin, Alireza Sadr, Yasunori Sumi, Junji Tagami

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13 Citations (Scopus)

Abstract

Objective To observe the bacterial demineralization of the enamel and dentin around composite restorations bonded with one-step and two-step self-etch adhesive systems using swept-source optical coherence tomography (SS-OCT). Methods Forty class V cavities (2.5-mm surface diameter, 2.0-mm maximum depth) were prepared on cervical areas of 20 human molars. The specimens were either treated with one-step adhesive (Clearfil Tri-S Bond ND Quick; TS) or two-step adhesive (Clearfil SE Bond; SE), restored with a flowable resin composite (Estelite Flow Quick). Specimens in the demineralized group were incubated for 2 weeks after Streptococcus mutans biofilm formation, while specimens in the control group were incubated for 2 weeks without biofilms. After SS-OCT observation, specimens were cut and examined under confocal laser scanning microscope (CLSM). The lesion depth (LD), interfacial gap depth (GD) and gap length (GL) obtained from SS-OCT and CLSM were analyzed by Pearson's correlation, LD by Independent-samples t-test, GD and GL by Welch t-test, the frequency of specimens with or without gap by Fisher's exact test at the 95% significant level. Results Specimens with interfacial gaps in the demineralized group showed significantly higher frequency than that in the control group (p < 0.05). There was a significant correlation between SS-OCT and CLSM values of LD, GD and GL (p < 0.05). SE showed significantly larger LD of dentin, but less GD and GL of enamel than TS (p < 0.05). Significance SS-OCT nondestructively detected demineralization around composite restorations and interfacial gaps created by S. mutans biofilm in this in vitro model.

Original languageEnglish
Pages (from-to)1177-1188
Number of pages12
JournalDental Materials
Volume32
Issue number9
DOIs
Publication statusPublished - Sep 1 2016
Externally publishedYes

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Keywords

  • Adhesive
  • Bacterial demineralization
  • Biofilm
  • Gap
  • Resin composite
  • Secondary caries
  • SS-OCT
  • Streptococcus mutans (S. mutans)

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

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