Assessment of bacterial demineralization around composite restorations using swept-source optical coherence tomography (SS-OCT)

Yuan Zhou, Yasushi Shimada, Khairul Matin, Alireza Sadr, Yasunori Sumi, Junji Tagami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective To observe the bacterial demineralization of the enamel and dentin around composite restorations bonded with one-step and two-step self-etch adhesive systems using swept-source optical coherence tomography (SS-OCT). Methods Forty class V cavities (2.5-mm surface diameter, 2.0-mm maximum depth) were prepared on cervical areas of 20 human molars. The specimens were either treated with one-step adhesive (Clearfil Tri-S Bond ND Quick; TS) or two-step adhesive (Clearfil SE Bond; SE), restored with a flowable resin composite (Estelite Flow Quick). Specimens in the demineralized group were incubated for 2 weeks after Streptococcus mutans biofilm formation, while specimens in the control group were incubated for 2 weeks without biofilms. After SS-OCT observation, specimens were cut and examined under confocal laser scanning microscope (CLSM). The lesion depth (LD), interfacial gap depth (GD) and gap length (GL) obtained from SS-OCT and CLSM were analyzed by Pearson's correlation, LD by Independent-samples t-test, GD and GL by Welch t-test, the frequency of specimens with or without gap by Fisher's exact test at the 95% significant level. Results Specimens with interfacial gaps in the demineralized group showed significantly higher frequency than that in the control group (p < 0.05). There was a significant correlation between SS-OCT and CLSM values of LD, GD and GL (p < 0.05). SE showed significantly larger LD of dentin, but less GD and GL of enamel than TS (p < 0.05). Significance SS-OCT nondestructively detected demineralization around composite restorations and interfacial gaps created by S. mutans biofilm in this in vitro model.

Original languageEnglish
Pages (from-to)1177-1188
Number of pages12
JournalDental Materials
Volume32
Issue number9
DOIs
Publication statusPublished - Sep 1 2016
Externally publishedYes

Fingerprint

Optical tomography
Optical Coherence Tomography
Restoration
Biofilms
Adhesives
Composite materials
Lasers
Microscopes
Streptococcus mutans
Enamels
Dentin
Dental Enamel
Scanning
Control Groups
Composite Resins
Resins
Observation

Keywords

  • Adhesive
  • Bacterial demineralization
  • Biofilm
  • Gap
  • Resin composite
  • Secondary caries
  • SS-OCT
  • Streptococcus mutans (S. mutans)

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Assessment of bacterial demineralization around composite restorations using swept-source optical coherence tomography (SS-OCT). / Zhou, Yuan; Shimada, Yasushi; Matin, Khairul; Sadr, Alireza; Sumi, Yasunori; Tagami, Junji.

In: Dental Materials, Vol. 32, No. 9, 01.09.2016, p. 1177-1188.

Research output: Contribution to journalArticle

Zhou, Yuan ; Shimada, Yasushi ; Matin, Khairul ; Sadr, Alireza ; Sumi, Yasunori ; Tagami, Junji. / Assessment of bacterial demineralization around composite restorations using swept-source optical coherence tomography (SS-OCT). In: Dental Materials. 2016 ; Vol. 32, No. 9. pp. 1177-1188.
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T1 - Assessment of bacterial demineralization around composite restorations using swept-source optical coherence tomography (SS-OCT)

AU - Zhou, Yuan

AU - Shimada, Yasushi

AU - Matin, Khairul

AU - Sadr, Alireza

AU - Sumi, Yasunori

AU - Tagami, Junji

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Objective To observe the bacterial demineralization of the enamel and dentin around composite restorations bonded with one-step and two-step self-etch adhesive systems using swept-source optical coherence tomography (SS-OCT). Methods Forty class V cavities (2.5-mm surface diameter, 2.0-mm maximum depth) were prepared on cervical areas of 20 human molars. The specimens were either treated with one-step adhesive (Clearfil Tri-S Bond ND Quick; TS) or two-step adhesive (Clearfil SE Bond; SE), restored with a flowable resin composite (Estelite Flow Quick). Specimens in the demineralized group were incubated for 2 weeks after Streptococcus mutans biofilm formation, while specimens in the control group were incubated for 2 weeks without biofilms. After SS-OCT observation, specimens were cut and examined under confocal laser scanning microscope (CLSM). The lesion depth (LD), interfacial gap depth (GD) and gap length (GL) obtained from SS-OCT and CLSM were analyzed by Pearson's correlation, LD by Independent-samples t-test, GD and GL by Welch t-test, the frequency of specimens with or without gap by Fisher's exact test at the 95% significant level. Results Specimens with interfacial gaps in the demineralized group showed significantly higher frequency than that in the control group (p < 0.05). There was a significant correlation between SS-OCT and CLSM values of LD, GD and GL (p < 0.05). SE showed significantly larger LD of dentin, but less GD and GL of enamel than TS (p < 0.05). Significance SS-OCT nondestructively detected demineralization around composite restorations and interfacial gaps created by S. mutans biofilm in this in vitro model.

AB - Objective To observe the bacterial demineralization of the enamel and dentin around composite restorations bonded with one-step and two-step self-etch adhesive systems using swept-source optical coherence tomography (SS-OCT). Methods Forty class V cavities (2.5-mm surface diameter, 2.0-mm maximum depth) were prepared on cervical areas of 20 human molars. The specimens were either treated with one-step adhesive (Clearfil Tri-S Bond ND Quick; TS) or two-step adhesive (Clearfil SE Bond; SE), restored with a flowable resin composite (Estelite Flow Quick). Specimens in the demineralized group were incubated for 2 weeks after Streptococcus mutans biofilm formation, while specimens in the control group were incubated for 2 weeks without biofilms. After SS-OCT observation, specimens were cut and examined under confocal laser scanning microscope (CLSM). The lesion depth (LD), interfacial gap depth (GD) and gap length (GL) obtained from SS-OCT and CLSM were analyzed by Pearson's correlation, LD by Independent-samples t-test, GD and GL by Welch t-test, the frequency of specimens with or without gap by Fisher's exact test at the 95% significant level. Results Specimens with interfacial gaps in the demineralized group showed significantly higher frequency than that in the control group (p < 0.05). There was a significant correlation between SS-OCT and CLSM values of LD, GD and GL (p < 0.05). SE showed significantly larger LD of dentin, but less GD and GL of enamel than TS (p < 0.05). Significance SS-OCT nondestructively detected demineralization around composite restorations and interfacial gaps created by S. mutans biofilm in this in vitro model.

KW - Adhesive

KW - Bacterial demineralization

KW - Biofilm

KW - Gap

KW - Resin composite

KW - Secondary caries

KW - SS-OCT

KW - Streptococcus mutans (S. mutans)

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