Assembly of the viroplasm by viral non-structural protein Pns10 is essential for persistent infection of rice ragged stunt virus in its insect vector

Dongsheng Jia, Nianmei Guo, Hongyan Chen, Fusamichi Akita, Lianhui Xie, Toshihiro Omura, Taiyun Wei

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Rice ragged stunt virus (RRSV), an oryzavirus, is transmitted by brown planthopper in a persistent propagative manner. In this study, sequential infection of RRSV in the internal organs of its insect vector after ingestion of virus was investigated by immunofluorescence microscopy. RRSV was first detected in the epithelial cells of the midgut, from where it proceeded to the visceral muscles surrounding the midgut, then throughout the visceral muscles of the midgut and hindgut, and finally into the salivary glands. Viroplasms, the sites of virus replication and assembly of progeny virions, were formed in the midgut epithelium, visceral muscles and salivary glands of infected insects and contained the non-structural protein Pns10 of RRSV, which appeared to be the major constituent of the viroplasms. Viroplasm-like structures formed in nonhost insect cells following expression of Pns10 in a baculovirus system, suggesting that the viroplasms observed in RRSV-infected cells were composed basically of Pns10. RNA interference induced by ingestion of dsRNA from the Pns10 gene of RRSV strongly inhibited such viroplasm formation, preventing efficient virus infection and spread in its insect vectors. These results show that Pns10 of RRSV is essential for viroplasm formation and virus replication in the vector insect.

Original languageEnglish
Pages (from-to)2299-2309
Number of pages11
JournalJournal of General Virology
Volume93
Issue numberPART 10
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

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Viral Nonstructural Proteins
Insect Vectors
Viruses
Infection
Virus Replication
Salivary Glands
Muscles
Insects
Eating
Reoviridae
Virus Assembly
Oryza
Baculoviridae
Virus Diseases
RNA Interference
Fluorescence Microscopy
Virion
Epithelium
Epithelial Cells

ASJC Scopus subject areas

  • Virology

Cite this

Assembly of the viroplasm by viral non-structural protein Pns10 is essential for persistent infection of rice ragged stunt virus in its insect vector. / Jia, Dongsheng; Guo, Nianmei; Chen, Hongyan; Akita, Fusamichi; Xie, Lianhui; Omura, Toshihiro; Wei, Taiyun.

In: Journal of General Virology, Vol. 93, No. PART 10, 10.2012, p. 2299-2309.

Research output: Contribution to journalArticle

Jia, Dongsheng ; Guo, Nianmei ; Chen, Hongyan ; Akita, Fusamichi ; Xie, Lianhui ; Omura, Toshihiro ; Wei, Taiyun. / Assembly of the viroplasm by viral non-structural protein Pns10 is essential for persistent infection of rice ragged stunt virus in its insect vector. In: Journal of General Virology. 2012 ; Vol. 93, No. PART 10. pp. 2299-2309.
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