Assays of PCB congeners and organochlorine insecticides with the transgenic Arabidopsis and tobacco plants carrying recombinant guinea pig AhR and GUS reporter genes

Keiko Gion, Hideyuki Inui, Hideaki Sasaki, Yasushi Utani, Susumu Kodama, Hideo Ohkawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g-1 PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.

Original languageEnglish
Pages (from-to)599-607
Number of pages9
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume47
Issue number7
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

Fingerprint

organochlorine insecticides
Insecticides
Tobacco
Glucuronidase
Polychlorinated Biphenyls
polychlorinated biphenyls
Polychlorinated biphenyls
Reporter Genes
Arabidopsis
reporter genes
guinea pigs
Assays
Guinea Pigs
tobacco
Genes
genetically modified organisms
assays
Ligands
Purification
Animals

Keywords

  • guinea pig aryl hydrocarbon receptor
  • organochlorine insecticide
  • Polychlorinated biphenyl
  • transgenic plants

ASJC Scopus subject areas

  • Food Science
  • Pollution

Cite this

@article{3501df1956534c958197c7005e25dca2,
title = "Assays of PCB congeners and organochlorine insecticides with the transgenic Arabidopsis and tobacco plants carrying recombinant guinea pig AhR and GUS reporter genes",
abstract = "Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g-1 PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.",
keywords = "guinea pig aryl hydrocarbon receptor, organochlorine insecticide, Polychlorinated biphenyl, transgenic plants",
author = "Keiko Gion and Hideyuki Inui and Hideaki Sasaki and Yasushi Utani and Susumu Kodama and Hideo Ohkawa",
year = "2012",
month = "8",
doi = "10.1080/03601234.2012.668453",
language = "English",
volume = "47",
pages = "599--607",
journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes",
issn = "0360-1234",
publisher = "Taylor and Francis Ltd.",
number = "7",

}

TY - JOUR

T1 - Assays of PCB congeners and organochlorine insecticides with the transgenic Arabidopsis and tobacco plants carrying recombinant guinea pig AhR and GUS reporter genes

AU - Gion, Keiko

AU - Inui, Hideyuki

AU - Sasaki, Hideaki

AU - Utani, Yasushi

AU - Kodama, Susumu

AU - Ohkawa, Hideo

PY - 2012/8

Y1 - 2012/8

N2 - Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g-1 PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.

AB - Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g-1 PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.

KW - guinea pig aryl hydrocarbon receptor

KW - organochlorine insecticide

KW - Polychlorinated biphenyl

KW - transgenic plants

UR - http://www.scopus.com/inward/record.url?scp=84861204954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861204954&partnerID=8YFLogxK

U2 - 10.1080/03601234.2012.668453

DO - 10.1080/03601234.2012.668453

M3 - Article

C2 - 22560022

AN - SCOPUS:84861204954

VL - 47

SP - 599

EP - 607

JO - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

JF - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

SN - 0360-1234

IS - 7

ER -