Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system

Hideyuki Inui, Keiko Gion, Yasushi Utani, Taketo Wakai, Susumu Kodama, Heesoo Eun, Yun Seok Kim, Hideo Ohkawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated β-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorodibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of life and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g -1 of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume47
Issue number4
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

Fingerprint

dioxins
Tobacco
Genetically Modified Plants
Glucuronidase
Reporter Genes
reporter genes
Gene expression
polluted soils
hydrocarbons
Assays
tobacco
Soil
Hydrocarbons
genetically modified organisms
Poisons
Soils
Gene Expression
gene expression
receptors
mice

Keywords

  • aryl hydrocarbon receptor
  • Biochemical assay
  • dioxin
  • polychlorinated biphenyl
  • transgenic plants

ASJC Scopus subject areas

  • Food Science
  • Pollution

Cite this

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title = "Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system",
abstract = "The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated β-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorodibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of life and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g -1 of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.",
keywords = "aryl hydrocarbon receptor, Biochemical assay, dioxin, polychlorinated biphenyl, transgenic plants",
author = "Hideyuki Inui and Keiko Gion and Yasushi Utani and Taketo Wakai and Susumu Kodama and Heesoo Eun and Kim, {Yun Seok} and Hideo Ohkawa",
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T1 - Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system

AU - Inui, Hideyuki

AU - Gion, Keiko

AU - Utani, Yasushi

AU - Wakai, Taketo

AU - Kodama, Susumu

AU - Eun, Heesoo

AU - Kim, Yun Seok

AU - Ohkawa, Hideo

PY - 2012/4

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