Ascorbic Acid Synergistically Potentiates Phloxine B-induced Photocytotoxicity in Human Acute Promyelocytic Leukemia Cells

Hang Qi, Qian Wu, Naomi Abe, Shunya Saiki, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ascorbic acid (AsA) is known as an antioxidant but concomitantly possesses a pro-oxidant property. Because the impact of AsA on photodynamic therapy response is unclear, we investigated the effect of AsA on photocytotoxicity induced by phloxine B in human acute promyelocytic leukemia HL-60 cells. AsA synergistically enhanced phloxine B-induced photocytotoxic effects, including inhibition of cell proliferation, DNA ladder formation, and caspase-3 activation, whereas AsA itself showed no photocytotoxicity. AsA also enhanced the consumption of the reduced glutathione level compared with the cells treated with phloxine B alone under the light condition. Combination of AsA with phloxine B under the light condition enhanced the phosphorylation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase (MAPK). These effects were completely cancelled by catalase. These results suggest that AsA synergistically enhances phloxine B-induced photocytotoxicity, possibly through the extracellular oxidative stress-dependent MAPK pathway activation.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalJournal of Biochemical and Molecular Toxicology
Volume28
Issue number4
DOIs
Publication statusPublished - Apr 2014

Keywords

  • Ascorbic Acid
  • HL-60 Cells
  • Hydrogen Peroxide
  • Phloxine B
  • Photocytotoxicity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

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