Ascorbate induced lipid peroxidation results in loss of receptor binding in Tris, but not in phosphate, buffer. Implications for the involvement of metal ions

Koji Abe, K. Kogure, H. Arai, M. Nakano

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Rat brain homogenate was incubated with various concentrations of ascorbate in a Tris-HCl buffer. Thiobarbituric acid-reactive substances (TBARS) were measured and 3H-QNB (quinuclidinyl benzilate) binding was also assayed on the same homogenate. Good parallelism between TBARS formation and loss of 3H-QNB binding activity confirmed that loss of 3H-QNB binding resulted from ascorbate-induced lipid peroxidation. However, neither formation of TBARS nor loss of 3H-QNB binding occurred in phosphate buffer or in the Tris-HCl system in the presence of metal-chelating reagents. This indicates that phosphate addition prevents the ascorbate effects due to complete chelation of intrinsic metal ions.

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalBiochemistry International
Volume11
Issue number3
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

Thiobarbituric Acid Reactive Substances
Lipid Peroxidation
Metal ions
Buffers
Metals
Phosphates
Ions
Chelation
Lipids
Quinuclidinyl Benzilate
Tromethamine
Rats
Brain

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{b219d800ce08414d904eec25d275db62,
title = "Ascorbate induced lipid peroxidation results in loss of receptor binding in Tris, but not in phosphate, buffer. Implications for the involvement of metal ions",
abstract = "Rat brain homogenate was incubated with various concentrations of ascorbate in a Tris-HCl buffer. Thiobarbituric acid-reactive substances (TBARS) were measured and 3H-QNB (quinuclidinyl benzilate) binding was also assayed on the same homogenate. Good parallelism between TBARS formation and loss of 3H-QNB binding activity confirmed that loss of 3H-QNB binding resulted from ascorbate-induced lipid peroxidation. However, neither formation of TBARS nor loss of 3H-QNB binding occurred in phosphate buffer or in the Tris-HCl system in the presence of metal-chelating reagents. This indicates that phosphate addition prevents the ascorbate effects due to complete chelation of intrinsic metal ions.",
author = "Koji Abe and K. Kogure and H. Arai and M. Nakano",
year = "1985",
language = "English",
volume = "11",
pages = "341--348",
journal = "IUBMB Life",
issn = "1521-6543",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Ascorbate induced lipid peroxidation results in loss of receptor binding in Tris, but not in phosphate, buffer. Implications for the involvement of metal ions

AU - Abe, Koji

AU - Kogure, K.

AU - Arai, H.

AU - Nakano, M.

PY - 1985

Y1 - 1985

N2 - Rat brain homogenate was incubated with various concentrations of ascorbate in a Tris-HCl buffer. Thiobarbituric acid-reactive substances (TBARS) were measured and 3H-QNB (quinuclidinyl benzilate) binding was also assayed on the same homogenate. Good parallelism between TBARS formation and loss of 3H-QNB binding activity confirmed that loss of 3H-QNB binding resulted from ascorbate-induced lipid peroxidation. However, neither formation of TBARS nor loss of 3H-QNB binding occurred in phosphate buffer or in the Tris-HCl system in the presence of metal-chelating reagents. This indicates that phosphate addition prevents the ascorbate effects due to complete chelation of intrinsic metal ions.

AB - Rat brain homogenate was incubated with various concentrations of ascorbate in a Tris-HCl buffer. Thiobarbituric acid-reactive substances (TBARS) were measured and 3H-QNB (quinuclidinyl benzilate) binding was also assayed on the same homogenate. Good parallelism between TBARS formation and loss of 3H-QNB binding activity confirmed that loss of 3H-QNB binding resulted from ascorbate-induced lipid peroxidation. However, neither formation of TBARS nor loss of 3H-QNB binding occurred in phosphate buffer or in the Tris-HCl system in the presence of metal-chelating reagents. This indicates that phosphate addition prevents the ascorbate effects due to complete chelation of intrinsic metal ions.

UR - http://www.scopus.com/inward/record.url?scp=0022416541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022416541&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 341

EP - 348

JO - IUBMB Life

JF - IUBMB Life

SN - 1521-6543

IS - 3

ER -