Rat brain homogenate was incubated with various concentrations of ascorbate in a Tris-HCl buffer. Thiobarbituric acid-reactive substances (TBARS) were measured and 3H-QNB (quinuclidinyl benzilate) binding was also assayed on the same homogenate. Good parallelism between TBARS formation and loss of 3H-QNB binding activity confirmed that loss of 3H-QNB binding resulted from ascorbate-induced lipid peroxidation. However, neither formation of TBARS nor loss of 3H-QNB binding occurred in phosphate buffer or in the Tris-HCl system in the presence of metal-chelating reagents. This indicates that phosphate addition prevents the ascorbate effects due to complete chelation of intrinsic metal ions.
|Number of pages||8|
|Publication status||Published - 1985|
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