Ascorbate-dependent enhancement of nitric oxide formation in activated macrophages

Akifumi Mizutani, Hideki Maki, Yasuyoshi Torii, Kiyotaka Hitomi, Norihiro Tsukagoshi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Macrophages activated with bacterial lipopolysaccharide (LPS) and cytokines produce nitric oxide through the induction of iNOS gene expression. Ascorbate increased NOx (nitrite and nitrate) formation by approximately 40% in a mouse macrophage-like cell line, J774.1, activated with LPS and interferon-γ. Ascorbate alone exhibited no inductive activity toward NO formation. N(G)-Monomethyl-L-arginine inhibited nitrite formation in cells activated in the presence or absence of ascorbate. Northern and Western blotting analyses showed that both iNOS mRNA and protein steady-state levels were increased approximately twofold in cells activated in the presence of ascorbate compared to in cells activated only with the inducers. These data suggest that ascorbate increased NO production by increasing the amount of iNOS in the activated macrophages.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalNitric Oxide - Biology and Chemistry
Volume2
Issue number4
DOIs
Publication statusPublished - Aug 1998
Externally publishedYes

Fingerprint

Macrophages
Nitric Oxide
Nitrites
Lipopolysaccharides
Gene expression
Northern Blotting
Nitrates
Interferons
Arginine
Western Blotting
Cells
Cytokines
Gene Expression
Cell Line
Messenger RNA
Proteins

Keywords

  • Ascorbate
  • Gene expression
  • Inducible nitric oxide synthase
  • Macrophage

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Ascorbate-dependent enhancement of nitric oxide formation in activated macrophages. / Mizutani, Akifumi; Maki, Hideki; Torii, Yasuyoshi; Hitomi, Kiyotaka; Tsukagoshi, Norihiro.

In: Nitric Oxide - Biology and Chemistry, Vol. 2, No. 4, 08.1998, p. 235-241.

Research output: Contribution to journalArticle

Mizutani, Akifumi ; Maki, Hideki ; Torii, Yasuyoshi ; Hitomi, Kiyotaka ; Tsukagoshi, Norihiro. / Ascorbate-dependent enhancement of nitric oxide formation in activated macrophages. In: Nitric Oxide - Biology and Chemistry. 1998 ; Vol. 2, No. 4. pp. 235-241.
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