Aryl hydrocarbon receptor (AhR)-mediated reporter gene expression systems in transgenic tobacco plants

Susumu Kodama, Kumiko Okada, Hideyuki Inui, Hideo Ohkawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In mammals, the aryl hydrocarbon receptor (AhR) mediates expression of certain genes, including CYP1A1, in response to exposure to dioxins and related compounds. We have constructed a mouse AhR-mediated gene expression systems for a β-glucuronidase (GUS) reporter gene consisting of an AhR, an AhR nuclear translocator (Arnt), and a xenobiotic response element (XRE)-driven promoter in transgenic tobacco plants. On treatment with the AhR ligands 3-methylcholanthrene (MC), β-naphthoflavone (βNF), and indigo, the transgenic tobacco plants exhibited enhanced GUS activity, presumably by inducible expression of the reporter gene. The recombinant AhR (AhRV), with the activation domain replaced by that of the Herpes simplex virus protein VP16, induced GUS activity much more than the wild-type AhR in the transgenic tobacco plants. Plants carrying AhRV expressed the GUS reporter gene in a dose- and time-dependent manner when treated with MC; GUS activity was detected at 5 nM MC on solid medium and at 12 h after soaking in 25 μM MC. Histochemical GUS staining showed that this system was active mainly in leaf and stem. These results suggest that the AhR-mediated reporter gene expression system has potential for the bioassay of dioxins in the environment and as a novel gene expression system in plants.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalPlanta
Volume227
Issue number1
DOIs
Publication statusPublished - Dec 1 2007

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Keywords

  • Aryl hydrocarbon receptor
  • Bioassay
  • Chemical-inducible
  • Dioxins
  • Gene expression
  • Transgenic plant

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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