Artemisinin

An alternative treatment for oral squamous cell carcinoma

Eiki Yamachika, Temesgen Habte, Dolphine Oda

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Artemisinin (AR) is a widely used antimalarial drug. Recently, additional uses for AR as an anticancer drug were discovered. Using TUNEL, immunohistochemistry (IHS) markers and flow cytometry techniques, we evaluated the effect of AR and 5-FU on HPV 16 immortalized and transformed human gingival epithelial (IHGK) cells. The results of TUNEL showed that AR-treated IHGK cells consisted of 82% positive cells, while 5-FU-treated cells consisted of 18% positive cells. The IHS markers demonstrated positive staining with Bax, p53, CD40 and CD40L in AR-treated cells and negative staining with Bcl-2. 5-FU-treated cells demonstrated a profile similar to AR but with less intensity. Cell cycle by flow cytometry results showed that only 5-FU-treated cells demonstrated a significant S-phase rate increase to 45%. In conclusion, our results indicate that AR is cytotoxic to transformed oral epithelial cells through apoptosis, while 5-FU is cytotoxic primarily through cell toxicity.

Original languageEnglish
Pages (from-to)2153-2160
Number of pages8
JournalAnticancer Research
Volume24
Issue number4
Publication statusPublished - Jul 2004

Fingerprint

Squamous Cell Carcinoma
Fluorouracil
In Situ Nick-End Labeling
Therapeutics
Flow Cytometry
Epithelial Cells
Immunohistochemistry
Negative Staining
CD40 Ligand
Human papillomavirus 16
artemisinine
Antimalarials
S Phase
Cell Cycle
Apoptosis
Staining and Labeling
Pharmaceutical Preparations

Keywords

  • Artemisinin
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Artemisinin : An alternative treatment for oral squamous cell carcinoma. / Yamachika, Eiki; Habte, Temesgen; Oda, Dolphine.

In: Anticancer Research, Vol. 24, No. 4, 07.2004, p. 2153-2160.

Research output: Contribution to journalArticle

Yamachika, Eiki ; Habte, Temesgen ; Oda, Dolphine. / Artemisinin : An alternative treatment for oral squamous cell carcinoma. In: Anticancer Research. 2004 ; Vol. 24, No. 4. pp. 2153-2160.
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