Arsenic (V) induces a fluidization of algal cell and liposome membranes

Le Quoc Tuan, Tran Thi Thanh Huong, Pham Thi Anh Hong, Tomonori Kawakami, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Arsenate is one of the most poisonous elements for living cells. When cells are exposed to arsenate, their life activities are immediately affected by various biochemical reactions, such as the binding of arsenic to membranes and the substitution of arsenic for phosphate or the choline head of phospholipids in the biological membranes. The effects of arsenate on the life activities of algae Chlorella vulgaris were investigated at various concentrations and exposure times. The results demonstrated that the living activities of algal cells (1010 cells/L) were seriously affected by arsenate at a concentration of more than 7.5 mg As/L within 24 h. Algal cells and the artificial membranes (liposomes) were exposed to arsenate to evaluate its effects on the membrane fluidization. In the presence of arsenate, the membranes were fluidized due to the binding and substitution of arsenate groups for phosphates or the choline head on the their membrane surface. This fluidization of the biological membranes was considered to enhance the transport of toxicants across the membrane of algal cells.

Original languageEnglish
Pages (from-to)1632-1638
Number of pages7
JournalToxicology in Vitro
Volume22
Issue number6
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Fingerprint

Fluidization
Arsenic
Liposomes
Cell Membrane
Membranes
Biological membranes
Phosphorylcholine
Choline
Substitution reactions
Phosphates
Cells
Chlorella vulgaris
Artificial Membranes
Algae
arsenic acid
Phospholipids

Keywords

  • Arsenate
  • Cell membrane
  • Chlorella vulgaris
  • Membrane fluidization
  • POPC liposomes

ASJC Scopus subject areas

  • Toxicology

Cite this

Tuan, L. Q., Huong, T. T. T., Hong, P. T. A., Kawakami, T., Shimanouchi, T., Umakoshi, H., & Kuboi, R. (2008). Arsenic (V) induces a fluidization of algal cell and liposome membranes. Toxicology in Vitro, 22(6), 1632-1638. https://doi.org/10.1016/j.tiv.2008.05.012

Arsenic (V) induces a fluidization of algal cell and liposome membranes. / Tuan, Le Quoc; Huong, Tran Thi Thanh; Hong, Pham Thi Anh; Kawakami, Tomonori; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Toxicology in Vitro, Vol. 22, No. 6, 09.2008, p. 1632-1638.

Research output: Contribution to journalArticle

Tuan, LQ, Huong, TTT, Hong, PTA, Kawakami, T, Shimanouchi, T, Umakoshi, H & Kuboi, R 2008, 'Arsenic (V) induces a fluidization of algal cell and liposome membranes', Toxicology in Vitro, vol. 22, no. 6, pp. 1632-1638. https://doi.org/10.1016/j.tiv.2008.05.012
Tuan, Le Quoc ; Huong, Tran Thi Thanh ; Hong, Pham Thi Anh ; Kawakami, Tomonori ; Shimanouchi, Toshinori ; Umakoshi, Hiroshi ; Kuboi, Ryoichi. / Arsenic (V) induces a fluidization of algal cell and liposome membranes. In: Toxicology in Vitro. 2008 ; Vol. 22, No. 6. pp. 1632-1638.
@article{bbb0710afad44a2ab6ae4a29f3c02d25,
title = "Arsenic (V) induces a fluidization of algal cell and liposome membranes",
abstract = "Arsenate is one of the most poisonous elements for living cells. When cells are exposed to arsenate, their life activities are immediately affected by various biochemical reactions, such as the binding of arsenic to membranes and the substitution of arsenic for phosphate or the choline head of phospholipids in the biological membranes. The effects of arsenate on the life activities of algae Chlorella vulgaris were investigated at various concentrations and exposure times. The results demonstrated that the living activities of algal cells (1010 cells/L) were seriously affected by arsenate at a concentration of more than 7.5 mg As/L within 24 h. Algal cells and the artificial membranes (liposomes) were exposed to arsenate to evaluate its effects on the membrane fluidization. In the presence of arsenate, the membranes were fluidized due to the binding and substitution of arsenate groups for phosphates or the choline head on the their membrane surface. This fluidization of the biological membranes was considered to enhance the transport of toxicants across the membrane of algal cells.",
keywords = "Arsenate, Cell membrane, Chlorella vulgaris, Membrane fluidization, POPC liposomes",
author = "Tuan, {Le Quoc} and Huong, {Tran Thi Thanh} and Hong, {Pham Thi Anh} and Tomonori Kawakami and Toshinori Shimanouchi and Hiroshi Umakoshi and Ryoichi Kuboi",
year = "2008",
month = "9",
doi = "10.1016/j.tiv.2008.05.012",
language = "English",
volume = "22",
pages = "1632--1638",
journal = "Toxicology in Vitro",
issn = "0887-2333",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Arsenic (V) induces a fluidization of algal cell and liposome membranes

AU - Tuan, Le Quoc

AU - Huong, Tran Thi Thanh

AU - Hong, Pham Thi Anh

AU - Kawakami, Tomonori

AU - Shimanouchi, Toshinori

AU - Umakoshi, Hiroshi

AU - Kuboi, Ryoichi

PY - 2008/9

Y1 - 2008/9

N2 - Arsenate is one of the most poisonous elements for living cells. When cells are exposed to arsenate, their life activities are immediately affected by various biochemical reactions, such as the binding of arsenic to membranes and the substitution of arsenic for phosphate or the choline head of phospholipids in the biological membranes. The effects of arsenate on the life activities of algae Chlorella vulgaris were investigated at various concentrations and exposure times. The results demonstrated that the living activities of algal cells (1010 cells/L) were seriously affected by arsenate at a concentration of more than 7.5 mg As/L within 24 h. Algal cells and the artificial membranes (liposomes) were exposed to arsenate to evaluate its effects on the membrane fluidization. In the presence of arsenate, the membranes were fluidized due to the binding and substitution of arsenate groups for phosphates or the choline head on the their membrane surface. This fluidization of the biological membranes was considered to enhance the transport of toxicants across the membrane of algal cells.

AB - Arsenate is one of the most poisonous elements for living cells. When cells are exposed to arsenate, their life activities are immediately affected by various biochemical reactions, such as the binding of arsenic to membranes and the substitution of arsenic for phosphate or the choline head of phospholipids in the biological membranes. The effects of arsenate on the life activities of algae Chlorella vulgaris were investigated at various concentrations and exposure times. The results demonstrated that the living activities of algal cells (1010 cells/L) were seriously affected by arsenate at a concentration of more than 7.5 mg As/L within 24 h. Algal cells and the artificial membranes (liposomes) were exposed to arsenate to evaluate its effects on the membrane fluidization. In the presence of arsenate, the membranes were fluidized due to the binding and substitution of arsenate groups for phosphates or the choline head on the their membrane surface. This fluidization of the biological membranes was considered to enhance the transport of toxicants across the membrane of algal cells.

KW - Arsenate

KW - Cell membrane

KW - Chlorella vulgaris

KW - Membrane fluidization

KW - POPC liposomes

UR - http://www.scopus.com/inward/record.url?scp=49149088016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49149088016&partnerID=8YFLogxK

U2 - 10.1016/j.tiv.2008.05.012

DO - 10.1016/j.tiv.2008.05.012

M3 - Article

C2 - 18577444

AN - SCOPUS:49149088016

VL - 22

SP - 1632

EP - 1638

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

IS - 6

ER -