Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A

Poonam R. Molli, Da Qiang Li, Rozita Bagheri-Yarmand, Suresh B. Pakala, Hiroshi Katayama, Subrata Sen, Jyoti Iyer, Jonathan Chernoff, Ming Ying Tsai, Sujit S. Nair, Rakesh Kumar

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Here we provide evidence in support of an inherent role for Arpc1b, a component of the Arp2/3 complex, in regulation of mitosis and demonstrate that its depletion inhibits Aurora A activation at the centrosome and impairs the ability of mammalian cells to enter mitosis. We discovered that Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on threonine 21, and expression of Arpc1b but not a nonphosphorylatable Arpc1b mutant in mammalian cells leads to Aurora A kinase activation and abnormal centrosome amplification in a Pak1-independent manner. Together, these findings reveal a new function for Arpc1b in centrosomal homeostasis. Arpc1b is both a physiological activator and substrate of Aurora A kinase and these interactions help to maintain mitotic integrity in mammalian cells.

Original languageEnglish
Pages (from-to)101-114
Number of pages14
JournalJournal of Cell Biology
Volume190
Issue number1
DOIs
Publication statusPublished - Jul 12 2010
Externally publishedYes

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Centrosome
Aurora Kinase A
Mitosis
Actin-Related Protein 2-3 Complex
Proteins
Threonine
Tubulin
Homeostasis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Molli, P. R., Li, D. Q., Bagheri-Yarmand, R., Pakala, S. B., Katayama, H., Sen, S., ... Kumar, R. (2010). Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Journal of Cell Biology, 190(1), 101-114. https://doi.org/10.1083/jcb.200908050

Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. / Molli, Poonam R.; Li, Da Qiang; Bagheri-Yarmand, Rozita; Pakala, Suresh B.; Katayama, Hiroshi; Sen, Subrata; Iyer, Jyoti; Chernoff, Jonathan; Tsai, Ming Ying; Nair, Sujit S.; Kumar, Rakesh.

In: Journal of Cell Biology, Vol. 190, No. 1, 12.07.2010, p. 101-114.

Research output: Contribution to journalArticle

Molli, PR, Li, DQ, Bagheri-Yarmand, R, Pakala, SB, Katayama, H, Sen, S, Iyer, J, Chernoff, J, Tsai, MY, Nair, SS & Kumar, R 2010, 'Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A', Journal of Cell Biology, vol. 190, no. 1, pp. 101-114. https://doi.org/10.1083/jcb.200908050
Molli, Poonam R. ; Li, Da Qiang ; Bagheri-Yarmand, Rozita ; Pakala, Suresh B. ; Katayama, Hiroshi ; Sen, Subrata ; Iyer, Jyoti ; Chernoff, Jonathan ; Tsai, Ming Ying ; Nair, Sujit S. ; Kumar, Rakesh. / Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. In: Journal of Cell Biology. 2010 ; Vol. 190, No. 1. pp. 101-114.
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