Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A

Poonam R. Molli, Da Qiang Li, Rozita Bagheri-Yarmand, Suresh B. Pakala, Hiroshi Katayama, Subrata Sen, Jyoti Iyer, Jonathan Chernoff, Ming Ying Tsai, Sujit S. Nair, Rakesh Kumar

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46 Citations (Scopus)

Abstract

Here we provide evidence in support of an inherent role for Arpc1b, a component of the Arp2/3 complex, in regulation of mitosis and demonstrate that its depletion inhibits Aurora A activation at the centrosome and impairs the ability of mammalian cells to enter mitosis. We discovered that Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on threonine 21, and expression of Arpc1b but not a nonphosphorylatable Arpc1b mutant in mammalian cells leads to Aurora A kinase activation and abnormal centrosome amplification in a Pak1-independent manner. Together, these findings reveal a new function for Arpc1b in centrosomal homeostasis. Arpc1b is both a physiological activator and substrate of Aurora A kinase and these interactions help to maintain mitotic integrity in mammalian cells.

Original languageEnglish
Pages (from-to)101-114
Number of pages14
JournalJournal of Cell Biology
Volume190
Issue number1
DOIs
Publication statusPublished - Jul 12 2010

ASJC Scopus subject areas

  • Cell Biology

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    Molli, P. R., Li, D. Q., Bagheri-Yarmand, R., Pakala, S. B., Katayama, H., Sen, S., Iyer, J., Chernoff, J., Tsai, M. Y., Nair, S. S., & Kumar, R. (2010). Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Journal of Cell Biology, 190(1), 101-114. https://doi.org/10.1083/jcb.200908050