Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling

Wulin Yang, Shermaine Thein, Chun Yan Lim, Russell E. Ericksen, Shigeki Sugii, Feng Xu, Robert C. Robinson, Jae Bum Kim, Weiping Han

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Extensive actin cytoskeleton remodelling occurs during adipocyte development. We have previously shown that disruption of stress fibres by the actin-severing protein cofilin is a requisite step in adipogenesis. However, it remains unclear whether actin nucleation and assembly into the cortical structure are essential for adipocyte development. In the present study we investigated the role of cortical actin assembly and of actin nucleation by the actin-related protein 2/3 (Arp2/3) complex in adipogenesis. Cortical actin structure formation started with accumulation of filamentous actin (F-actin) patches near the plasma membrane during adipogenesis. Depletion of Arp2/3 by knockdown of its subunits Arp3 or ARPC3 strongly impaired adipocyte differentiation, although adipogenesis-initiating factors were unaffected. Moreover, the assembly of F-actin-rich structures at the plasma membrane was suppressed and the cortical actin structure poorly developed after adipogenic induction in Arp2/3-deficient cells. Finally, we provide evidence that the cortical actin cytoskeleton is essential for efficient glucose transporter 4 (GLUT4) vesicle exocytosis and insulin signal transduction. These results show that the Arp2/3 complex is an essential regulator of adipocyte development through control of the formation of cortical actin structures,which may facilitate nutrient uptake and signalling events.

Original languageEnglish
Pages (from-to)179-192
Number of pages14
JournalBiochemical Journal
Volume464
Issue number2
DOIs
Publication statusPublished - Dec 1 2014
Externally publishedYes

Fingerprint

Actin-Related Protein 2-3 Complex
Adipogenesis
Actins
Adipocytes
Actin-Related Protein 2
Actin-Related Protein 3
Actin Cytoskeleton
Cell membranes
Cell Membrane
Actin Depolymerizing Factors
Nucleation
Stress Fibers
Facilitative Glucose Transport Proteins
Exocytosis
Signal transduction
Signal Transduction
Nutrients
Insulin

Keywords

  • Actin remodelling
  • Actin-related protein 2/3 (Arp2/3)
  • Adipogenesis
  • Insulin signalling
  • Nucleation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yang, W., Thein, S., Lim, C. Y., Ericksen, R. E., Sugii, S., Xu, F., ... Han, W. (2014). Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling. Biochemical Journal, 464(2), 179-192. https://doi.org/10.1042/BJ20140805

Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling. / Yang, Wulin; Thein, Shermaine; Lim, Chun Yan; Ericksen, Russell E.; Sugii, Shigeki; Xu, Feng; Robinson, Robert C.; Kim, Jae Bum; Han, Weiping.

In: Biochemical Journal, Vol. 464, No. 2, 01.12.2014, p. 179-192.

Research output: Contribution to journalArticle

Yang, W, Thein, S, Lim, CY, Ericksen, RE, Sugii, S, Xu, F, Robinson, RC, Kim, JB & Han, W 2014, 'Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling', Biochemical Journal, vol. 464, no. 2, pp. 179-192. https://doi.org/10.1042/BJ20140805
Yang W, Thein S, Lim CY, Ericksen RE, Sugii S, Xu F et al. Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling. Biochemical Journal. 2014 Dec 1;464(2):179-192. https://doi.org/10.1042/BJ20140805
Yang, Wulin ; Thein, Shermaine ; Lim, Chun Yan ; Ericksen, Russell E. ; Sugii, Shigeki ; Xu, Feng ; Robinson, Robert C. ; Kim, Jae Bum ; Han, Weiping. / Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling. In: Biochemical Journal. 2014 ; Vol. 464, No. 2. pp. 179-192.
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