Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning

Akinori Okumura; Masanori Sano; Takehiro Suzuki; Hiroyasu Tanaka; Ryo Nagao; Katsuyoshi Nakazato; Masako Iwai; Hideyuki Adachi; Jian Ren Shen; Isao Enami

doi:10.1016/j.febslet.2007.10.015

Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning

Akinori Okumura, Masanori Sano, Takehiro Suzuki, Hiroyasu Tanaka, Ryo Nagao, Katsuyoshi Nakazato, Masako Iwai, Hideyuki Adachi, Jian Ren Shen, Isao Enami

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca²⁺ and Cl^- cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants with the red algal PSII. The results revealed that Tyr-92 of PsbU, especially its aromatic ring, was essential for maintaining its function. From the crystal structure of PSII, Tyr-92 is located close to Pro-340 of D1, suggesting that the aromatic ring of Tyr-92 interacts with the CH group of Pro-340 of D1, and this CH/π interaction is important for the optimal function of the Mn₄Ca-cluster.

Original language	English
Pages (from-to)	5255-5258
Number of pages	4
Journal	FEBS Letters
Volume	581
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2007.10.015
Publication status	Published - Nov 13 2007

Keywords

Extrinsic protein
Oxygen evolution
Photosystem II
PsbU
Red algae

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.10.015

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title = "Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning",

abstract = "PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca2+ and Cl- cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants with the red algal PSII. The results revealed that Tyr-92 of PsbU, especially its aromatic ring, was essential for maintaining its function. From the crystal structure of PSII, Tyr-92 is located close to Pro-340 of D1, suggesting that the aromatic ring of Tyr-92 interacts with the CH group of Pro-340 of D1, and this CH/π interaction is important for the optimal function of the Mn4Ca-cluster.",

keywords = "Extrinsic protein, Oxygen evolution, Photosystem II, PsbU, Red algae",

author = "Akinori Okumura and Masanori Sano and Takehiro Suzuki and Hiroyasu Tanaka and Ryo Nagao and Katsuyoshi Nakazato and Masako Iwai and Hideyuki Adachi and Shen, {Jian Ren} and Isao Enami",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Technology of Japan (MEXT) to I.E. (18570049) and Scientific Research on Priority Areas from the MEXT to K.N. and J.-R.S. (16087102).",

year = "2007",

month = nov,

day = "13",

doi = "10.1016/j.febslet.2007.10.015",

language = "English",

volume = "581",

pages = "5255--5258",

journal = "FEBS Letters",

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number = "27",

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TY - JOUR

T1 - Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning

AU - Okumura, Akinori

AU - Sano, Masanori

AU - Suzuki, Takehiro

AU - Tanaka, Hiroyasu

AU - Nagao, Ryo

AU - Nakazato, Katsuyoshi

AU - Iwai, Masako

AU - Adachi, Hideyuki

AU - Shen, Jian Ren

AU - Enami, Isao

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Technology of Japan (MEXT) to I.E. (18570049) and Scientific Research on Priority Areas from the MEXT to K.N. and J.-R.S. (16087102).

PY - 2007/11/13

Y1 - 2007/11/13

N2 - PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca2+ and Cl- cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants with the red algal PSII. The results revealed that Tyr-92 of PsbU, especially its aromatic ring, was essential for maintaining its function. From the crystal structure of PSII, Tyr-92 is located close to Pro-340 of D1, suggesting that the aromatic ring of Tyr-92 interacts with the CH group of Pro-340 of D1, and this CH/π interaction is important for the optimal function of the Mn4Ca-cluster.

AB - PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca2+ and Cl- cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants with the red algal PSII. The results revealed that Tyr-92 of PsbU, especially its aromatic ring, was essential for maintaining its function. From the crystal structure of PSII, Tyr-92 is located close to Pro-340 of D1, suggesting that the aromatic ring of Tyr-92 interacts with the CH group of Pro-340 of D1, and this CH/π interaction is important for the optimal function of the Mn4Ca-cluster.

KW - Extrinsic protein

KW - Oxygen evolution

KW - Photosystem II

KW - PsbU

KW - Red algae

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JO - FEBS Letters

JF - FEBS Letters

IS - 27

ER -

Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning

Abstract

Keywords

ASJC Scopus subject areas

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