Arg tyrosine kinase is involved in homologous recombinational DNA repair

Yingzhu Li, Hiroko Shimizu, Shuang Lin Xiang, Yoshiro Maru, Noriaki Takao, Ken-ichi Yamamoto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume299
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

abl Genes
Recombinational DNA Repair
Repair
Genes
Ionizing radiation
Ionizing Radiation
DNA
DNA Damage
HEK293 Cells
Automatic teller machines
Aberrations
Chromosome Aberrations
DNA Repair
Chickens
Hypersensitivity
B-Lymphocytes
Cells
ARG tyrosine kinase
Cell Line

Keywords

  • c-Abl family
  • Double-strand break
  • Homologous recombinational DNA repair
  • Rad51 focus
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Arg tyrosine kinase is involved in homologous recombinational DNA repair. / Li, Yingzhu; Shimizu, Hiroko; Xiang, Shuang Lin; Maru, Yoshiro; Takao, Noriaki; Yamamoto, Ken-ichi.

In: Biochemical and Biophysical Research Communications, Vol. 299, No. 5, 2002, p. 697-702.

Research output: Contribution to journalArticle

Li, Yingzhu ; Shimizu, Hiroko ; Xiang, Shuang Lin ; Maru, Yoshiro ; Takao, Noriaki ; Yamamoto, Ken-ichi. / Arg tyrosine kinase is involved in homologous recombinational DNA repair. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 299, No. 5. pp. 697-702.
@article{f3f940e95bb44aa5b0c910ff08a5c498,
title = "Arg tyrosine kinase is involved in homologous recombinational DNA repair",
abstract = "c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.",
keywords = "c-Abl family, Double-strand break, Homologous recombinational DNA repair, Rad51 focus, Tyrosine phosphorylation",
author = "Yingzhu Li and Hiroko Shimizu and Xiang, {Shuang Lin} and Yoshiro Maru and Noriaki Takao and Ken-ichi Yamamoto",
year = "2002",
doi = "10.1016/S0006-291X(02)02692-X",
language = "English",
volume = "299",
pages = "697--702",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Arg tyrosine kinase is involved in homologous recombinational DNA repair

AU - Li, Yingzhu

AU - Shimizu, Hiroko

AU - Xiang, Shuang Lin

AU - Maru, Yoshiro

AU - Takao, Noriaki

AU - Yamamoto, Ken-ichi

PY - 2002

Y1 - 2002

N2 - c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

AB - c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

KW - c-Abl family

KW - Double-strand break

KW - Homologous recombinational DNA repair

KW - Rad51 focus

KW - Tyrosine phosphorylation

UR - http://www.scopus.com/inward/record.url?scp=0036921395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036921395&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(02)02692-X

DO - 10.1016/S0006-291X(02)02692-X

M3 - Article

C2 - 12470634

AN - SCOPUS:0036921395

VL - 299

SP - 697

EP - 702

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 5

ER -