Abstract
c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.
Original language | English |
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Pages (from-to) | 697-702 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 299 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2002 |
Externally published | Yes |
Keywords
- Double-strand break
- Homologous recombinational DNA repair
- Rad51 focus
- Tyrosine phosphorylation
- c-Abl family
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology