Arg tyrosine kinase is involved in homologous recombinational DNA repair

Yingzhu Li; Hiroko Shimizu; Shuang Lin Xiang; Yoshiro Maru; Noriaki Takao; Ken Ichi Yamamoto

doi:10.1016/S0006-291X(02)02692-X

Arg tyrosine kinase is involved in homologous recombinational DNA repair

Yingzhu Li, Hiroko Shimizu, Shuang Lin Xiang, Yoshiro Maru, Noriaki Takao, Ken Ichi Yamamoto

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

Original language	English
Pages (from-to)	697-702
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	299
Issue number	5
DOIs	https://doi.org/10.1016/S0006-291X(02)02692-X
Publication status	Published - 2002
Externally published	Yes

Keywords

Double-strand break
Homologous recombinational DNA repair
Rad51 focus
Tyrosine phosphorylation
c-Abl family

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{f3f940e95bb44aa5b0c910ff08a5c498,

title = "Arg tyrosine kinase is involved in homologous recombinational DNA repair",

abstract = "c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.",

keywords = "Double-strand break, Homologous recombinational DNA repair, Rad51 focus, Tyrosine phosphorylation, c-Abl family",

author = "Yingzhu Li and Hiroko Shimizu and Xiang, {Shuang Lin} and Yoshiro Maru and Noriaki Takao and Yamamoto, {Ken Ichi}",

note = "Funding Information: We thank Y. Shiloh for discussion and for performing the ATM phosphorylation experiments, S. Takeda for discussion, K. Yoshioka for the JSAP2 targeting vector, and A. Shinohara for human Rad51 cDNA and the antibody to human Rad51. This work is supported by in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.",

year = "2002",

doi = "10.1016/S0006-291X(02)02692-X",

language = "English",

volume = "299",

pages = "697--702",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "5",

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TY - JOUR

T1 - Arg tyrosine kinase is involved in homologous recombinational DNA repair

AU - Li, Yingzhu

AU - Shimizu, Hiroko

AU - Xiang, Shuang Lin

AU - Maru, Yoshiro

AU - Takao, Noriaki

AU - Yamamoto, Ken Ichi

N1 - Funding Information: We thank Y. Shiloh for discussion and for performing the ATM phosphorylation experiments, S. Takeda for discussion, K. Yoshioka for the JSAP2 targeting vector, and A. Shinohara for human Rad51 cDNA and the antibody to human Rad51. This work is supported by in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.

PY - 2002

Y1 - 2002

N2 - c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

AB - c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

KW - Double-strand break

KW - Homologous recombinational DNA repair

KW - Rad51 focus

KW - Tyrosine phosphorylation

KW - c-Abl family

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