Arg tyrosine kinase is involved in homologous recombinational DNA repair

Yingzhu Li, Hiroko Shimizu, Shuang Lin Xiang, Yoshiro Maru, Noriaki Takao, Ken Ichi Yamamoto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume299
Issue number5
DOIs
Publication statusPublished - Dec 1 2002

Keywords

  • Double-strand break
  • Homologous recombinational DNA repair
  • Rad51 focus
  • Tyrosine phosphorylation
  • c-Abl family

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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