ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Iγ

Michael Krauss, Masahiro Kinuta, Markus R. Wenk, Pietro De Camilli, Kohji Takei, Volker Haucke

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPγS on clathrin coat recruitment is mediated at least in part by increased levels of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Iγ (PIPKIγ), in this effect. These data suggest a model according to which activation of PIPKIγ by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.

Original languageEnglish
Pages (from-to)113-124
Number of pages12
JournalJournal of Cell Biology
Volume162
Issue number1
DOIs
Publication statusPublished - Jul 7 2003

Fingerprint

Phosphatidylinositol Phosphates
Synaptic Membranes
Clathrin
Phosphotransferases
Synaptic Vesicles
Phosphatidylinositols
Endocytosis
Guanosine Triphosphate
Synapses
Adenosine Triphosphate
Cell Membrane
ADP-ribosylation factor 6
Proteins
1-phosphatidylinositol-4-phosphate 5-kinase

Keywords

  • ARF
  • Clathrin coat assembly
  • Endocytosis
  • PIP formation
  • PIPK Iγ

ASJC Scopus subject areas

  • Cell Biology

Cite this

ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Iγ. / Krauss, Michael; Kinuta, Masahiro; Wenk, Markus R.; De Camilli, Pietro; Takei, Kohji; Haucke, Volker.

In: Journal of Cell Biology, Vol. 162, No. 1, 07.07.2003, p. 113-124.

Research output: Contribution to journalArticle

Krauss, Michael ; Kinuta, Masahiro ; Wenk, Markus R. ; De Camilli, Pietro ; Takei, Kohji ; Haucke, Volker. / ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Iγ. In: Journal of Cell Biology. 2003 ; Vol. 162, No. 1. pp. 113-124.
@article{f24b3791725d4d26bb846cd8805d9a86,
title = "ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Iγ",
abstract = "Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPγS on clathrin coat recruitment is mediated at least in part by increased levels of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Iγ (PIPKIγ), in this effect. These data suggest a model according to which activation of PIPKIγ by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.",
keywords = "ARF, Clathrin coat assembly, Endocytosis, PIP formation, PIPK Iγ",
author = "Michael Krauss and Masahiro Kinuta and Wenk, {Markus R.} and {De Camilli}, Pietro and Kohji Takei and Volker Haucke",
year = "2003",
month = "7",
day = "7",
doi = "10.1083/jcb.200301006",
language = "English",
volume = "162",
pages = "113--124",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Iγ

AU - Krauss, Michael

AU - Kinuta, Masahiro

AU - Wenk, Markus R.

AU - De Camilli, Pietro

AU - Takei, Kohji

AU - Haucke, Volker

PY - 2003/7/7

Y1 - 2003/7/7

N2 - Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPγS on clathrin coat recruitment is mediated at least in part by increased levels of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Iγ (PIPKIγ), in this effect. These data suggest a model according to which activation of PIPKIγ by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.

AB - Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPγS on clathrin coat recruitment is mediated at least in part by increased levels of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Iγ (PIPKIγ), in this effect. These data suggest a model according to which activation of PIPKIγ by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.

KW - ARF

KW - Clathrin coat assembly

KW - Endocytosis

KW - PIP formation

KW - PIPK Iγ

UR - http://www.scopus.com/inward/record.url?scp=0038825177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038825177&partnerID=8YFLogxK

U2 - 10.1083/jcb.200301006

DO - 10.1083/jcb.200301006

M3 - Article

C2 - 12847086

AN - SCOPUS:0038825177

VL - 162

SP - 113

EP - 124

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -