Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size

Akira Shinaoka, Ryusuke Momota, Eri Shiratsuchi, Mitsuko Kosaka, Kanae Kumagishi, Ryuuichi Nakahara, Ichiro Naito, Aiji Ohtsuka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules; the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels.

Original languageEnglish
Pages (from-to)406-414
Number of pages9
JournalMicroscopy and Microanalysis
Volume19
Issue number2
DOIs
Publication statusPublished - Apr 2013

Fingerprint

blood vessels
Blood vessels
vessels
fibers
Fibers
Hemodynamics
arterioles
mesh
hemodynamics
Elastin
Formic acid
elastin
resilience
Rats
Structural properties
formic acid
quantitative analysis
rats
casts
Scanning electron microscopy

Keywords

  • elastic fiber
  • elastin
  • formic acid digestion
  • rat
  • SEM
  • three-dimensional architecture
  • vascular corrosion casting

ASJC Scopus subject areas

  • Instrumentation

Cite this

@article{96709877de594eb2a231053fa082e294,
title = "Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size",
abstract = "Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules; the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels.",
keywords = "elastic fiber, elastin, formic acid digestion, rat, SEM, three-dimensional architecture, vascular corrosion casting",
author = "Akira Shinaoka and Ryusuke Momota and Eri Shiratsuchi and Mitsuko Kosaka and Kanae Kumagishi and Ryuuichi Nakahara and Ichiro Naito and Aiji Ohtsuka",
year = "2013",
month = "4",
doi = "10.1017/S1431927612014341",
language = "English",
volume = "19",
pages = "406--414",
journal = "Microscopy and Microanalysis",
issn = "1431-9276",
publisher = "Cambridge University Press",
number = "2",

}

TY - JOUR

T1 - Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size

AU - Shinaoka, Akira

AU - Momota, Ryusuke

AU - Shiratsuchi, Eri

AU - Kosaka, Mitsuko

AU - Kumagishi, Kanae

AU - Nakahara, Ryuuichi

AU - Naito, Ichiro

AU - Ohtsuka, Aiji

PY - 2013/4

Y1 - 2013/4

N2 - Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules; the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels.

AB - Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules; the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels.

KW - elastic fiber

KW - elastin

KW - formic acid digestion

KW - rat

KW - SEM

KW - three-dimensional architecture

KW - vascular corrosion casting

UR - http://www.scopus.com/inward/record.url?scp=84875475989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875475989&partnerID=8YFLogxK

U2 - 10.1017/S1431927612014341

DO - 10.1017/S1431927612014341

M3 - Article

C2 - 23453051

AN - SCOPUS:84875475989

VL - 19

SP - 406

EP - 414

JO - Microscopy and Microanalysis

JF - Microscopy and Microanalysis

SN - 1431-9276

IS - 2

ER -