Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone

Akihiro C.E. Shibata, Takahiro K. Fujiwara, Limin Chen, Kenichi G.N. Suzuki, Yoshiro Ishikawa, Yuri L. Nemoto, Yoshihiro Miwa, Ziya Kalay, Rahul Chadda, Keiji Naruse, Akihiro Kusumi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The focal adhesion (FA) is an integrin-based structure built in/on the plasma membrane, mechanically linking the extracellular matrix with the termini of actin stress fibers, providing key scaffolds for the cells to migrate in tissues. The FA was considered as a micron-scale, massive assembly of various proteins, although its formation and decomposition occur quickly in several to several 10 s of minutes. The mechanism of rapid FA regulation has been a major mystery in cell biology. Here, using fast single fluorescent-molecule imaging, we found that transferrin receptor and Thy1, non-FA membrane proteins, readily enter the FA zone, diffuse rapidly there, and exit into the bulk plasma membrane. Integrin β3 also readily enters the FA zone, and repeatedly undergoes temporary immobilization and diffusion in the FA zone, whereas approximately one-third of integrin β3 is immobilized there. These results are consistent with the archipelago architecture of the FA, which consists of many integrin islands: the membrane molecules enter the inter-island channels rather freely, and the integrins in the integrin islands can be rapidly exchanged with those in the bulk membrane. Such an archipelago architecture would allow rapid FA formation and disintegration, and might be applicable to other large protein domains in the plasma membrane.

Original languageEnglish
Pages (from-to)380-392
Number of pages13
JournalCytoskeleton
Volume69
Issue number6
DOIs
Publication statusPublished - 2012

Fingerprint

Focal Adhesions
Integrins
Membranes
Cell Membrane
Islands
Channel Islands
Stress Fibers
Transferrin Receptors
Immobilization
Extracellular Matrix
Cell Biology
Actins
Membrane Proteins

Keywords

  • GPI-anchored protein
  • Integrin
  • Single fluorescent-molecule imaging/tracking
  • Thy1
  • Transferrin receptor
  • Transmembrane protein

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

Cite this

Shibata, A. C. E., Fujiwara, T. K., Chen, L., Suzuki, K. G. N., Ishikawa, Y., Nemoto, Y. L., ... Kusumi, A. (2012). Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone. Cytoskeleton, 69(6), 380-392. https://doi.org/10.1002/cm.21032

Archipelago architecture of the focal adhesion : Membrane molecules freely enter and exit from the focal adhesion zone. / Shibata, Akihiro C.E.; Fujiwara, Takahiro K.; Chen, Limin; Suzuki, Kenichi G.N.; Ishikawa, Yoshiro; Nemoto, Yuri L.; Miwa, Yoshihiro; Kalay, Ziya; Chadda, Rahul; Naruse, Keiji; Kusumi, Akihiro.

In: Cytoskeleton, Vol. 69, No. 6, 2012, p. 380-392.

Research output: Contribution to journalArticle

Shibata, ACE, Fujiwara, TK, Chen, L, Suzuki, KGN, Ishikawa, Y, Nemoto, YL, Miwa, Y, Kalay, Z, Chadda, R, Naruse, K & Kusumi, A 2012, 'Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone', Cytoskeleton, vol. 69, no. 6, pp. 380-392. https://doi.org/10.1002/cm.21032
Shibata, Akihiro C.E. ; Fujiwara, Takahiro K. ; Chen, Limin ; Suzuki, Kenichi G.N. ; Ishikawa, Yoshiro ; Nemoto, Yuri L. ; Miwa, Yoshihiro ; Kalay, Ziya ; Chadda, Rahul ; Naruse, Keiji ; Kusumi, Akihiro. / Archipelago architecture of the focal adhesion : Membrane molecules freely enter and exit from the focal adhesion zone. In: Cytoskeleton. 2012 ; Vol. 69, No. 6. pp. 380-392.
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