Arabidopsis proteins with a transposon-related domain act in gene silencing

Yoko Ikeda; Thierry Pélissier; Pierre Bourguet; Claude Becker; Marie Noëlle Pouch-Pélissier; Romain Pogorelcnik; Magdalena Weingartner; Detlef Weigel; Jean Marc Deragon; Olivier Mathieu

doi:10.1038/ncomms15122

Arabidopsis proteins with a transposon-related domain act in gene silencing

Yoko Ikeda, Thierry Pélissier, Pierre Bourguet, Claude Becker, Marie Noëlle Pouch-Pélissier, Romain Pogorelcnik, Magdalena Weingartner, Detlef Weigel, Jean Marc Deragon, Olivier Mathieu

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposonrelated plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.

Original language	English
Article number	15122
Journal	Nature communications
Volume	8
DOIs	https://doi.org/10.1038/ncomms15122
Publication status	Published - 2017

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/ncomms15122

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@article{472ffe4005174933bfd8505c02aeacd0,

title = "Arabidopsis proteins with a transposon-related domain act in gene silencing",

abstract = "Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposonrelated plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.",

author = "Yoko Ikeda and Thierry P{\'e}lissier and Pierre Bourguet and Claude Becker and Pouch-P{\'e}lissier, {Marie No{\"e}lle} and Romain Pogorelcnik and Magdalena Weingartner and Detlef Weigel and Deragon, {Jean Marc} and Olivier Mathieu",

note = "Funding Information: We thank L. L{\'o}pez-Gonz{\'a}lez (Universit{\'e} Clermont Auvergne) and M. Fujii (Okayama University) for technical assistance, T. Hirayama (Okayama University) for experimental support and helpful discussions, and N. Suzuki (Okayama University) for support with confocal microscopy analysis. This work was supported by a grant from the European Research Council (ERC, I2ST 260742 to O.M.), an EMBO Young Investigator award (to O.M.), a Young Researcher grant from the Auvergne Regional Council (to O.M.), and the Max Planck Society and DFG (SFB 1101 to D.W.). Y.I. was supported by the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship for research abroad, a Grant-in-Aid for Research Activity Start-up (26891018) and for Young Scientists B (15K18578), and Program to Disseminate Tenure Tracking System, MEXT, Japan. P.B. was supported by a PhD studentship from the Minist{\`e}re de l'{\'e}ducation nationale, de l'enseignement sup{\'e}rieur et de la recherche. Funding Information: We thank L. L{\'o}pez-Gonz{\'a}lez (Universit{\'e} Clermont Auvergne) and M. Fujii (Okayama University) for technical assistance, T. Hirayama (Okayama University) for experimental support and helpful discussions, and N. Suzuki (Okayama University) for support with confocal microscopy analysis. This work was supported by a grant from the European Research Council (ERC, I2ST 260742 to O.M.), an EMBO Young Investigator award (to O.M.), a Young Researcher grant from the Auvergne Regional Council (to O.M.), and the Max Planck Society and DFG (SFB 1101 to D.W.). Y.I. was supported by the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship for research abroad, a Grant-in-Aid for Research Activity Start-up (26891018) and for Young Scientists B (15K18578), and Program to Disseminate Tenure Tracking System, MEXT, Japan. P.B. was supported by a PhD studentship from the Minist{\`e}re de l{\textquoteright}{\'e}ducation nationale, de l{\textquoteright}enseignement sup{\'e}rieur et de la recherche. Publisher Copyright: {\textcopyright} The Author(s) 2017.",

year = "2017",

doi = "10.1038/ncomms15122",

language = "English",

volume = "8",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - Arabidopsis proteins with a transposon-related domain act in gene silencing

AU - Ikeda, Yoko

AU - Pélissier, Thierry

AU - Bourguet, Pierre

AU - Becker, Claude

AU - Pouch-Pélissier, Marie Noëlle

AU - Pogorelcnik, Romain

AU - Weingartner, Magdalena

AU - Weigel, Detlef

AU - Deragon, Jean Marc

AU - Mathieu, Olivier

N1 - Funding Information: We thank L. López-González (Université Clermont Auvergne) and M. Fujii (Okayama University) for technical assistance, T. Hirayama (Okayama University) for experimental support and helpful discussions, and N. Suzuki (Okayama University) for support with confocal microscopy analysis. This work was supported by a grant from the European Research Council (ERC, I2ST 260742 to O.M.), an EMBO Young Investigator award (to O.M.), a Young Researcher grant from the Auvergne Regional Council (to O.M.), and the Max Planck Society and DFG (SFB 1101 to D.W.). Y.I. was supported by the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship for research abroad, a Grant-in-Aid for Research Activity Start-up (26891018) and for Young Scientists B (15K18578), and Program to Disseminate Tenure Tracking System, MEXT, Japan. P.B. was supported by a PhD studentship from the Ministère de l'éducation nationale, de l'enseignement supérieur et de la recherche. Funding Information: We thank L. López-González (Université Clermont Auvergne) and M. Fujii (Okayama University) for technical assistance, T. Hirayama (Okayama University) for experimental support and helpful discussions, and N. Suzuki (Okayama University) for support with confocal microscopy analysis. This work was supported by a grant from the European Research Council (ERC, I2ST 260742 to O.M.), an EMBO Young Investigator award (to O.M.), a Young Researcher grant from the Auvergne Regional Council (to O.M.), and the Max Planck Society and DFG (SFB 1101 to D.W.). Y.I. was supported by the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship for research abroad, a Grant-in-Aid for Research Activity Start-up (26891018) and for Young Scientists B (15K18578), and Program to Disseminate Tenure Tracking System, MEXT, Japan. P.B. was supported by a PhD studentship from the Ministère de l’éducation nationale, de l’enseignement supérieur et de la recherche. Publisher Copyright: © The Author(s) 2017.

PY - 2017

Y1 - 2017

N2 - Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposonrelated plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.

AB - Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposonrelated plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.

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U2 - 10.1038/ncomms15122

DO - 10.1038/ncomms15122

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JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 15122

ER -

Arabidopsis proteins with a transposon-related domain act in gene silencing

Abstract

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