TY - JOUR
T1 - Arabidopsis proteins with a transposon-related domain act in gene silencing
AU - Ikeda, Yoko
AU - Pélissier, Thierry
AU - Bourguet, Pierre
AU - Becker, Claude
AU - Pouch-Pélissier, Marie Noëlle
AU - Pogorelcnik, Romain
AU - Weingartner, Magdalena
AU - Weigel, Detlef
AU - Deragon, Jean Marc
AU - Mathieu, Olivier
N1 - Funding Information:
We thank L. López-González (Université Clermont Auvergne) and M. Fujii (Okayama University) for technical assistance, T. Hirayama (Okayama University) for experimental support and helpful discussions, and N. Suzuki (Okayama University) for support with confocal microscopy analysis. This work was supported by a grant from the European Research Council (ERC, I2ST 260742 to O.M.), an EMBO Young Investigator award (to O.M.), a Young Researcher grant from the Auvergne Regional Council (to O.M.), and the Max Planck Society and DFG (SFB 1101 to D.W.). Y.I. was supported by the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship for research abroad, a Grant-in-Aid for Research Activity Start-up (26891018) and for Young Scientists B (15K18578), and Program to Disseminate Tenure Tracking System, MEXT, Japan. P.B. was supported by a PhD studentship from the Ministère de l'éducation nationale, de l'enseignement supérieur et de la recherche.
Funding Information:
We thank L. López-González (Université Clermont Auvergne) and M. Fujii (Okayama University) for technical assistance, T. Hirayama (Okayama University) for experimental support and helpful discussions, and N. Suzuki (Okayama University) for support with confocal microscopy analysis. This work was supported by a grant from the European Research Council (ERC, I2ST 260742 to O.M.), an EMBO Young Investigator award (to O.M.), a Young Researcher grant from the Auvergne Regional Council (to O.M.), and the Max Planck Society and DFG (SFB 1101 to D.W.). Y.I. was supported by the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship for research abroad, a Grant-in-Aid for Research Activity Start-up (26891018) and for Young Scientists B (15K18578), and Program to Disseminate Tenure Tracking System, MEXT, Japan. P.B. was supported by a PhD studentship from the Ministère de l’éducation nationale, de l’enseignement supérieur et de la recherche.
Publisher Copyright:
© The Author(s) 2017.
PY - 2017
Y1 - 2017
N2 - Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposonrelated plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.
AB - Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposonrelated plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.
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U2 - 10.1038/ncomms15122
DO - 10.1038/ncomms15122
M3 - Article
C2 - 28466841
AN - SCOPUS:85033673000
VL - 8
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 15122
ER -