Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination

Chengwei Liu, Tatsuya Fukumoto, Tadashi Matsumoto, Patrizia Gena, Daniele Frascaria, Tomoyuki Kaneko, Maki Katsuhara, Shihua Zhong, Xiaoli Sun, Yanming Zhu, Ikuko Iwasaki, Xiaodong Ding, Giuseppe Calamita, Yoshichika Kitagawa

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

OsPIP1;1 is one of the most abundant aquaporins in rice leaves and roots and is highly responsible to environmental stresses. However, its biochemical and physiological functions are still largely unknown. The oocyte assay data showed OsPIP1;1 had lower water channel activity in contrast to OsPIP2;1. EGFP and immunoelectron microscopy studies revealed OsPIP1;1 was predominantly localized in not only plasma membrane but also in some ER-like intracellular compartments in the cells. OsPIP1;1 exhibited low water channel activity in Xenopus oocytes but coexpression of OsPIP2;1 significantly enhanced its water permeability. Stop-flow assay indicated that 10His-OsPIP1;1-reconstituted proteoliposomes had significantly higher water permeability than the control liposomes. Overexpression of OsPIP1;1 greatly altered many physiological features of transgenic plants in a dosage-dependent manner. Moderate expression of OsPIP1;1 increased rice seed yield, salt resistance, root hydraulic conductivity, and seed germination rate. This work suggests OsPIP1;1 functions as an active water channel and plays important physiological roles.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalPlant Physiology and Biochemistry
Volume63
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Aquaporin 1
Aquaporins
aquaporins
Germination
Seeds
seed germination
Salts
salts
rice
Oocytes
Permeability
water
permeability
oocytes
Immunoelectron Microscopy
Water
Genetically Modified Plants
root hydraulic conductivity
Xenopus
Liposomes

Keywords

  • Aquaporin
  • Cellular localization
  • Salt resistance
  • Seed germination
  • Seed yield
  • Water permeability

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Liu, C., Fukumoto, T., Matsumoto, T., Gena, P., Frascaria, D., Kaneko, T., ... Kitagawa, Y. (2013). Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination. Plant Physiology and Biochemistry, 63, 151-158. https://doi.org/10.1016/j.plaphy.2012.11.018

Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination. / Liu, Chengwei; Fukumoto, Tatsuya; Matsumoto, Tadashi; Gena, Patrizia; Frascaria, Daniele; Kaneko, Tomoyuki; Katsuhara, Maki; Zhong, Shihua; Sun, Xiaoli; Zhu, Yanming; Iwasaki, Ikuko; Ding, Xiaodong; Calamita, Giuseppe; Kitagawa, Yoshichika.

In: Plant Physiology and Biochemistry, Vol. 63, 02.2013, p. 151-158.

Research output: Contribution to journalArticle

Liu, C, Fukumoto, T, Matsumoto, T, Gena, P, Frascaria, D, Kaneko, T, Katsuhara, M, Zhong, S, Sun, X, Zhu, Y, Iwasaki, I, Ding, X, Calamita, G & Kitagawa, Y 2013, 'Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination', Plant Physiology and Biochemistry, vol. 63, pp. 151-158. https://doi.org/10.1016/j.plaphy.2012.11.018
Liu, Chengwei ; Fukumoto, Tatsuya ; Matsumoto, Tadashi ; Gena, Patrizia ; Frascaria, Daniele ; Kaneko, Tomoyuki ; Katsuhara, Maki ; Zhong, Shihua ; Sun, Xiaoli ; Zhu, Yanming ; Iwasaki, Ikuko ; Ding, Xiaodong ; Calamita, Giuseppe ; Kitagawa, Yoshichika. / Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination. In: Plant Physiology and Biochemistry. 2013 ; Vol. 63. pp. 151-158.
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