Abstract
We tried to clarify the applicability of a single prolonged stress (SPS) protocol as post-traumatic stress disorder (PTSD) model in mice. To investigate PTSD pathophysiology, we conducted hypothalamo-pituitary-adrenal (HPA) negative feedback testing at 1, 4, 8 and 12 weeks after the SPS by administrating a dexamethasone (DEX) suppression test. The SPS induced over-suppression of the HPA system by DEX treatment at 8 and 12 weeks. To investigate PTSD-like behavioral characteristics, we subjected mice to testing in a light/dark box (to assess anxiety), a Y-maze (working memory), a cliff avoidance (visual cognition), and an open field (locomotor activity) at 1, 4, 8 and 12 weeks after the SPS. In the light/dark box test, the SPS-applied mice spent significantly less time in the light box at 8 or 12 weeks. In the cliff avoidance test, the SPS-applied mice spent significantly less time in the open area at 1 week. However, in both the Y-maze test and the open field test, SPS-applied mice tended toward slight decreases in a time-dependent manner until 12 weeks. Therefore, SPS-applied mice may thus be useful for assessing characteristics relevant to PTSD that coincide with changes in the HPA axis.
| Original language | English |
|---|---|
| Pages (from-to) | 479-486 |
| Number of pages | 8 |
| Journal | Acta Medica Okayama |
| Volume | 72 |
| Issue number | 5 |
| Publication status | Published - Jan 1 2018 |
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Keywords
- Corticosterone
- Mouse
- PTSD
- Single prolonged stress
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Application of single prolonged stress induces post-traumatic stress disorder-like characteristics in mice. / Tanaka, Ken Ichi; Yagi, Takao; Nanba, Takeshi; Asanuma, Masato.
In: Acta Medica Okayama, Vol. 72, No. 5, 01.01.2018, p. 479-486.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of single prolonged stress induces post-traumatic stress disorder-like characteristics in mice
AU - Tanaka, Ken Ichi
AU - Yagi, Takao
AU - Nanba, Takeshi
AU - Asanuma, Masato
PY - 2018/1/1
Y1 - 2018/1/1
N2 - We tried to clarify the applicability of a single prolonged stress (SPS) protocol as post-traumatic stress disorder (PTSD) model in mice. To investigate PTSD pathophysiology, we conducted hypothalamo-pituitary-adrenal (HPA) negative feedback testing at 1, 4, 8 and 12 weeks after the SPS by administrating a dexamethasone (DEX) suppression test. The SPS induced over-suppression of the HPA system by DEX treatment at 8 and 12 weeks. To investigate PTSD-like behavioral characteristics, we subjected mice to testing in a light/dark box (to assess anxiety), a Y-maze (working memory), a cliff avoidance (visual cognition), and an open field (locomotor activity) at 1, 4, 8 and 12 weeks after the SPS. In the light/dark box test, the SPS-applied mice spent significantly less time in the light box at 8 or 12 weeks. In the cliff avoidance test, the SPS-applied mice spent significantly less time in the open area at 1 week. However, in both the Y-maze test and the open field test, SPS-applied mice tended toward slight decreases in a time-dependent manner until 12 weeks. Therefore, SPS-applied mice may thus be useful for assessing characteristics relevant to PTSD that coincide with changes in the HPA axis.
AB - We tried to clarify the applicability of a single prolonged stress (SPS) protocol as post-traumatic stress disorder (PTSD) model in mice. To investigate PTSD pathophysiology, we conducted hypothalamo-pituitary-adrenal (HPA) negative feedback testing at 1, 4, 8 and 12 weeks after the SPS by administrating a dexamethasone (DEX) suppression test. The SPS induced over-suppression of the HPA system by DEX treatment at 8 and 12 weeks. To investigate PTSD-like behavioral characteristics, we subjected mice to testing in a light/dark box (to assess anxiety), a Y-maze (working memory), a cliff avoidance (visual cognition), and an open field (locomotor activity) at 1, 4, 8 and 12 weeks after the SPS. In the light/dark box test, the SPS-applied mice spent significantly less time in the light box at 8 or 12 weeks. In the cliff avoidance test, the SPS-applied mice spent significantly less time in the open area at 1 week. However, in both the Y-maze test and the open field test, SPS-applied mice tended toward slight decreases in a time-dependent manner until 12 weeks. Therefore, SPS-applied mice may thus be useful for assessing characteristics relevant to PTSD that coincide with changes in the HPA axis.
KW - Corticosterone
KW - Mouse
KW - PTSD
KW - Single prolonged stress
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UR - http://www.scopus.com/inward/citedby.url?scp=85055614688&partnerID=8YFLogxK
M3 - Article
VL - 72
SP - 479
EP - 486
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 5
ER -