Application of low temperatures during photoinhibition allows characterization of individual steps in photodamage and the repair of photosystem II

Prasanna Mohanty, Suleyman I. Allakhverdiev, Norio Murata

    Research output: Contribution to journalReview articlepeer-review

    71 Citations (Scopus)

    Abstract

    Recent investigations of photoinhibition have revealed that photodamage to photosystem II (PSII) involves two temporally separated steps: the first is the inactivation of the oxygen-evolving complex by light that has been absorbed by the manganese cluster and the second is the impairment of the photochemical reaction center by light that has been absorbed by chlorophyll. Our studies of photoinhibition in Synechocystis sp. PCC 6803 at various temperatures demonstrated that the first step in photodamage is not completed at low temperatures, such as 10°C. Further investigations suggested that an intermediate state, which is stabilized at low temperatures, might exist at the first stage of photodamage. The repair of PSII involves many steps, including degradation and removal of the D1 protein, synthesis de novo of the precursor to the D1 protein, assembly of the PSII complex, and processing of the precursor to the D1 protein. Detailed analysis of photodamage and repair at various temperatures has demonstrated that, among these steps, only the synthesis of the precursor to D1 appears to proceed at low temperatures. Investigations of photoinhibition at low temperatures have also indicated that prolonged exposure of cyanobacterial cells or plant leaves to strong light diminishes their ability to repair PSII. Such non-repairable photoinhibition is caused by inhibition of the processing of the precursor to the D1 protein after prolonged illumination with strong light at low temperatures.

    Original languageEnglish
    Pages (from-to)217-224
    Number of pages8
    JournalPhotosynthesis research
    Volume94
    Issue number2-3
    DOIs
    Publication statusPublished - Nov 2007

    Keywords

    • D1 protein
    • Low temperature
    • Photodamage
    • Photoinhibition
    • Photosynthesis
    • Photosystem II
    • Processing
    • Repair

    ASJC Scopus subject areas

    • Biochemistry
    • Plant Science
    • Cell Biology

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