Application of hydroxyapatite nanoparticle-assembled powder using basic fibroblast growth factor as a pulp-capping agent

Kazuki Imura, Yoshiya Hashimoto, Masahiro Okada, Kazushi Yoshikawa, Kazuyo Yamamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)


We have previously fabricated hydroxyapatite (HAP) nanoparticle-assembled powder (nano-HAP) plates and granules by assembling low-crystallinity HAP nanoparticles without template/binder molecules or high-temperature/pressure treatments. In this study, we combined the nano-HAP with fibroblast growth factor (FGF) 2, which promotes odontoblast differentiation, and used this as a pulp-capping agent for dentin defects created in rat molars. The tissue response was then radiologically and histologically assessed at 1 and 2 weeks after capping, to assess the biocompatibility and ability of this material to promote hard tissue formation. The application of nano-HAP/FGF2 induced the invasion of dental pulp cells and vessels, and was consistently found to stimulate formation of a dentinal bridge containing numerous dentinal tubules. We thus succeeded in treating the pulp exposure by using a physiological approach to promote tissue regeneration. Further investigations should be performed to explain exactly how the nano-HAP/FGF2 combination contributes to calcified tissue formation.

Original languageEnglish
Pages (from-to)713-720
Number of pages8
JournalDental materials journal
Issue number5
Publication statusPublished - Jan 1 2019



  • Basic fibroblast growth factor
  • Hydroxyapatite
  • Nanoparticle-assembled powder
  • Pulp-capping agen

ASJC Scopus subject areas

  • Ceramics and Composites
  • Dentistry(all)

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