Application of Hadamard transformation to MEKC

Kazuki Hata, Takashi Kaneta, Totaro Imasaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Hadamard transform (HT) technique, which permits the S/N in CE to be improved, was applied to MEKC. Multiple sample injection of fluorescent analytes according to a Hadamard code sequence was performed using an optically gated sample injection technique, in which a sample plug was produced based on photodegradation by irradiation with an intense laser beam. The capillary and reservoirs were filled with a sample solution containing buffer components and SDS as a pseudostationary phase. A preliminary study confirmed that fluorescein ion could be photobleached in the presence of SDS. The optically gated sample injection technique was then applied to multiple sample injection, based on a Hadamard matrix. The S/N in the electropherogram obtained by HT-MEKC was improved substantially compared to that obtained by a single injection method. When the technique was applied to the separation of several amino acids labeled with FITC, the S/N ratio for each amino acid was enhanced, without any evidence of degradation in separation resolution. Moreover, HT-MEKC was applied to the analysis of amino acids contained in a Japanese beverage, resulting in improved S/Ns for the amino acids.

Original languageEnglish
Pages (from-to)328-334
Number of pages7
JournalElectrophoresis
Volume28
Issue number3
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

Fingerprint

Micellar Electrokinetic Capillary Chromatography
Hadamard transforms
Amino Acids
Injections
Hadamard matrices
Beverages
Fluorescein-5-isothiocyanate
Photodegradation
Fluorescein
Photolysis
Laser beams
Buffers
Irradiation
Ions
Lasers
Degradation

Keywords

  • Hadamard transformation
  • MEKC
  • Multiple sample injection

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Application of Hadamard transformation to MEKC. / Hata, Kazuki; Kaneta, Takashi; Imasaka, Totaro.

In: Electrophoresis, Vol. 28, No. 3, 02.2007, p. 328-334.

Research output: Contribution to journalArticle

Hata, Kazuki ; Kaneta, Takashi ; Imasaka, Totaro. / Application of Hadamard transformation to MEKC. In: Electrophoresis. 2007 ; Vol. 28, No. 3. pp. 328-334.
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