Abstract
Background: Enamel matrix derivative (EMD) is a tissue regenerative agent used clinically as an adjunct to periodontal surgery. It was previously demonstrated that Porphyromonas gingivalis, a periodontal pathogen, significantly diminished the efficacy of EMD with periodontal ligament (PDL) cells through the proteolytic actions of Arg- and Lys-gingipains (Rgp and Kgp). Thus, antiproteolytic supplements are considered clinically desirable for effective periodontal regenerative therapies. In the present study, we examined apple- (AP) and hop-polyphenols to determine their ability to protect EMD-stimulated PDL cells from P. gingivalis. Methods: AP, apple condensed tannin (ACT), hop bract polyphenol (HBP), high and low molecular weight fractions of HBP (HMW-HBP and LMW-HBP), and epigallocatechin gallate (EGCg) were used. PDL cells were grown on EMD-coated dishes and infected with P. gingivalis, and cellular migration and proliferation were evaluated with an in vitro assay of wound heating assay in the presence or absence of the polyphenols. Results: Each polyphenol significantly enhanced the viability of PDL cells infected with P. gingivalis, whereas only EGCg demonstrated cytotoxicity. Further, all polyphenols significantly inhibited Rgp activity, with AP, ACT, and HBP more effective toward Kgp. P. gingivalis markedly diminished the migration and proliferation of EMD-stimulated PDL cells, whereas the addition of AP, ACT, HBP, and HMW-HBP significantly protected the cells from bacterial cytotoxicity. In contrast, EGCg and LMW-HBP did not show protective effects. Conclusion: These results suggest that AP, ACT, AP, HBP, and HMW-HBP protect EMD-stimulated PDL cells from P. gingivalis and i-nay be therapeutically useful supplements for EMD therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 2223-2229 |
| Number of pages | 7 |
| Journal | Journal of Periodontology |
| Volume | 76 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2005 |
| Externally published | Yes |
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Keywords
- Enamel
- Periodontal ligament
- Polyphenols
- Porphyromonas gingivalis
- Therapy
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis. / Inaba, Hiroaki; Tagashira, Motoyuki; Kanda, Tomomasa; Ohno, Takashi; Kawai, Shinji; Amano, Atsuo.
In: Journal of Periodontology, Vol. 76, No. 12, 12.2005, p. 2223-2229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis
AU - Inaba, Hiroaki
AU - Tagashira, Motoyuki
AU - Kanda, Tomomasa
AU - Ohno, Takashi
AU - Kawai, Shinji
AU - Amano, Atsuo
PY - 2005/12
Y1 - 2005/12
N2 - Background: Enamel matrix derivative (EMD) is a tissue regenerative agent used clinically as an adjunct to periodontal surgery. It was previously demonstrated that Porphyromonas gingivalis, a periodontal pathogen, significantly diminished the efficacy of EMD with periodontal ligament (PDL) cells through the proteolytic actions of Arg- and Lys-gingipains (Rgp and Kgp). Thus, antiproteolytic supplements are considered clinically desirable for effective periodontal regenerative therapies. In the present study, we examined apple- (AP) and hop-polyphenols to determine their ability to protect EMD-stimulated PDL cells from P. gingivalis. Methods: AP, apple condensed tannin (ACT), hop bract polyphenol (HBP), high and low molecular weight fractions of HBP (HMW-HBP and LMW-HBP), and epigallocatechin gallate (EGCg) were used. PDL cells were grown on EMD-coated dishes and infected with P. gingivalis, and cellular migration and proliferation were evaluated with an in vitro assay of wound heating assay in the presence or absence of the polyphenols. Results: Each polyphenol significantly enhanced the viability of PDL cells infected with P. gingivalis, whereas only EGCg demonstrated cytotoxicity. Further, all polyphenols significantly inhibited Rgp activity, with AP, ACT, and HBP more effective toward Kgp. P. gingivalis markedly diminished the migration and proliferation of EMD-stimulated PDL cells, whereas the addition of AP, ACT, HBP, and HMW-HBP significantly protected the cells from bacterial cytotoxicity. In contrast, EGCg and LMW-HBP did not show protective effects. Conclusion: These results suggest that AP, ACT, AP, HBP, and HMW-HBP protect EMD-stimulated PDL cells from P. gingivalis and i-nay be therapeutically useful supplements for EMD therapy.
AB - Background: Enamel matrix derivative (EMD) is a tissue regenerative agent used clinically as an adjunct to periodontal surgery. It was previously demonstrated that Porphyromonas gingivalis, a periodontal pathogen, significantly diminished the efficacy of EMD with periodontal ligament (PDL) cells through the proteolytic actions of Arg- and Lys-gingipains (Rgp and Kgp). Thus, antiproteolytic supplements are considered clinically desirable for effective periodontal regenerative therapies. In the present study, we examined apple- (AP) and hop-polyphenols to determine their ability to protect EMD-stimulated PDL cells from P. gingivalis. Methods: AP, apple condensed tannin (ACT), hop bract polyphenol (HBP), high and low molecular weight fractions of HBP (HMW-HBP and LMW-HBP), and epigallocatechin gallate (EGCg) were used. PDL cells were grown on EMD-coated dishes and infected with P. gingivalis, and cellular migration and proliferation were evaluated with an in vitro assay of wound heating assay in the presence or absence of the polyphenols. Results: Each polyphenol significantly enhanced the viability of PDL cells infected with P. gingivalis, whereas only EGCg demonstrated cytotoxicity. Further, all polyphenols significantly inhibited Rgp activity, with AP, ACT, and HBP more effective toward Kgp. P. gingivalis markedly diminished the migration and proliferation of EMD-stimulated PDL cells, whereas the addition of AP, ACT, HBP, and HMW-HBP significantly protected the cells from bacterial cytotoxicity. In contrast, EGCg and LMW-HBP did not show protective effects. Conclusion: These results suggest that AP, ACT, AP, HBP, and HMW-HBP protect EMD-stimulated PDL cells from P. gingivalis and i-nay be therapeutically useful supplements for EMD therapy.
KW - Enamel
KW - Periodontal ligament
KW - Polyphenols
KW - Porphyromonas gingivalis
KW - Therapy
UR - http://www.scopus.com/inward/record.url?scp=30944464285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30944464285&partnerID=8YFLogxK
U2 - 10.1902/jop.2005.76.12.2223
DO - 10.1902/jop.2005.76.12.2223
M3 - Article
C2 - 16332233
AN - SCOPUS:30944464285
VL - 76
SP - 2223
EP - 2229
JO - Journal of Periodontology
JF - Journal of Periodontology
SN - 0022-3492
IS - 12
ER -