Abstract
Autolysis easily happens to sea cucumber (Stichopus japonicus, S. japonicus) for external stimulus like UV exposure causing heavy economic losses. Therefore, it is meaningful to reveal the mechanism of S. japonicas autolysis. In the present study, to examine the involvement of apoptosis induction in UVA-induced autolysis of S. japonicas, we investigated the biochemical events including the DNA fragmentation, caspase-3 activation, mitogen-activated protein kinases (MAPKs) phosphorylation and free radical formation. Substantial morphological changes such as intestine vomiting and dermatolysis were observed in S. japonicus during the incubation after 1-h UVA irradiation (10 W/m2). The degradation of the structural proteins and enhancement of cathepsin L activity were also detected, suggesting the profound impact of proteolysis caused by the UVA irradiation even for 1 h. Furthermore, the DNA fragmentation and specific activity of caspase-3 was increased up to 12 h after UVA irradiation. The levels of phosphorylated p38 mitogen activated protein kinase (MAPK) and phosphorylated c-Jun.-N-terminal kinase (JNK) were significantly increased by the UVA irradiation for 1 h. An electron spin resonance (ESR) analysis revealed that UVA enhanced the free radical formation in S. japonicas, even through we could not identify the attributed species. These results suggest that UVA-induced autolysis in S. japonicas at least partially involves the oxidative stress-sensitive apoptosis induction pathway. These data present a novel insight into the mechanisms of sea cucumber autolysis induced by external stress.
| Original language | English |
|---|---|
| Pages (from-to) | 130-135 |
| Number of pages | 6 |
| Journal | Journal of Photochemistry and Photobiology B: Biology |
| Volume | 158 |
| DOIs | |
| Publication status | Published - May 1 2016 |
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Keywords
- Apoptosis
- Autolysis
- Free radical
- MAPK phosphorylation
- Sea cucumber Stichopus japonicus
- UVA
ASJC Scopus subject areas
- Radiation
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
- Biophysics
Cite this
Apoptosis induction is involved in UVA-induced autolysis in sea cucumber Stichopus japonicus. / Qi, Hang; Fu, Hui; Dong, Xiufang; Feng, Dingding; Li, Nan; Wen, Chengrong; Nakamura, Yoshimasa; Zhu, Beiwei.
In: Journal of Photochemistry and Photobiology B: Biology, Vol. 158, 01.05.2016, p. 130-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptosis induction is involved in UVA-induced autolysis in sea cucumber Stichopus japonicus
AU - Qi, Hang
AU - Fu, Hui
AU - Dong, Xiufang
AU - Feng, Dingding
AU - Li, Nan
AU - Wen, Chengrong
AU - Nakamura, Yoshimasa
AU - Zhu, Beiwei
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Autolysis easily happens to sea cucumber (Stichopus japonicus, S. japonicus) for external stimulus like UV exposure causing heavy economic losses. Therefore, it is meaningful to reveal the mechanism of S. japonicas autolysis. In the present study, to examine the involvement of apoptosis induction in UVA-induced autolysis of S. japonicas, we investigated the biochemical events including the DNA fragmentation, caspase-3 activation, mitogen-activated protein kinases (MAPKs) phosphorylation and free radical formation. Substantial morphological changes such as intestine vomiting and dermatolysis were observed in S. japonicus during the incubation after 1-h UVA irradiation (10 W/m2). The degradation of the structural proteins and enhancement of cathepsin L activity were also detected, suggesting the profound impact of proteolysis caused by the UVA irradiation even for 1 h. Furthermore, the DNA fragmentation and specific activity of caspase-3 was increased up to 12 h after UVA irradiation. The levels of phosphorylated p38 mitogen activated protein kinase (MAPK) and phosphorylated c-Jun.-N-terminal kinase (JNK) were significantly increased by the UVA irradiation for 1 h. An electron spin resonance (ESR) analysis revealed that UVA enhanced the free radical formation in S. japonicas, even through we could not identify the attributed species. These results suggest that UVA-induced autolysis in S. japonicas at least partially involves the oxidative stress-sensitive apoptosis induction pathway. These data present a novel insight into the mechanisms of sea cucumber autolysis induced by external stress.
AB - Autolysis easily happens to sea cucumber (Stichopus japonicus, S. japonicus) for external stimulus like UV exposure causing heavy economic losses. Therefore, it is meaningful to reveal the mechanism of S. japonicas autolysis. In the present study, to examine the involvement of apoptosis induction in UVA-induced autolysis of S. japonicas, we investigated the biochemical events including the DNA fragmentation, caspase-3 activation, mitogen-activated protein kinases (MAPKs) phosphorylation and free radical formation. Substantial morphological changes such as intestine vomiting and dermatolysis were observed in S. japonicus during the incubation after 1-h UVA irradiation (10 W/m2). The degradation of the structural proteins and enhancement of cathepsin L activity were also detected, suggesting the profound impact of proteolysis caused by the UVA irradiation even for 1 h. Furthermore, the DNA fragmentation and specific activity of caspase-3 was increased up to 12 h after UVA irradiation. The levels of phosphorylated p38 mitogen activated protein kinase (MAPK) and phosphorylated c-Jun.-N-terminal kinase (JNK) were significantly increased by the UVA irradiation for 1 h. An electron spin resonance (ESR) analysis revealed that UVA enhanced the free radical formation in S. japonicas, even through we could not identify the attributed species. These results suggest that UVA-induced autolysis in S. japonicas at least partially involves the oxidative stress-sensitive apoptosis induction pathway. These data present a novel insight into the mechanisms of sea cucumber autolysis induced by external stress.
KW - Apoptosis
KW - Autolysis
KW - Free radical
KW - MAPK phosphorylation
KW - Sea cucumber Stichopus japonicus
KW - UVA
UR - http://www.scopus.com/inward/record.url?scp=84960131206&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84960131206&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2016.02.034
DO - 10.1016/j.jphotobiol.2016.02.034
M3 - Article
VL - 158
SP - 130
EP - 135
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
ER -