Apoptosis in glomerular sclerosis

Hitoshi Sugiyama, Naoki Kashihara, Hirofumi Makino, Yasushi Yamasaki, Zensuke Ota

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Glomerulosclerosis is characterized by progressive extracellular matrix accumulation and glomerular cell loss. The role of glomerular cell apoptosis in glomerulosclerosis was investigated in the rat remnant kidney model and in human glomerular diseases. We identified apoptotic cells in the glomeruli, tubules and interstitium in the remnant kidney by electron microscopy. DNA fragmentation, which is a biochemical characteristic of apoptosis, was detected by in situ nick end-labeling of fragmented DNA with terminal deoxynucleotidyl transferase and biotinylated deoxyuridine triphosphate. Fragmented DNA in the glomeruli and tubules increased with the progression of glomerulosclerosis in the remnant kidney model. This finding was also demonstrated in other glomerular sclerotic lesions such as IgA and lupus nephritis. The number of cells positive for nick end-labeling in the glomerulus significantly correlated with the degree of glomerulosclerosis and the deterioration of renal function. These results indicate that apoptosis is, at least in part, involved in the cell deletion of various glomerular diseases leading to sclerosis.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalKidney International
Volume49
Issue number1
Publication statusPublished - 1996

Fingerprint

Sclerosis
Apoptosis
Kidney
Lupus Nephritis
DNA Nucleotidylexotransferase
DNA
In Situ Nick-End Labeling
DNA Fragmentation
Immunoglobulin A
Extracellular Matrix
Electron Microscopy
Cell Count

ASJC Scopus subject areas

  • Nephrology

Cite this

Apoptosis in glomerular sclerosis. / Sugiyama, Hitoshi; Kashihara, Naoki; Makino, Hirofumi; Yamasaki, Yasushi; Ota, Zensuke.

In: Kidney International, Vol. 49, No. 1, 1996, p. 103-111.

Research output: Contribution to journalArticle

Sugiyama, H, Kashihara, N, Makino, H, Yamasaki, Y & Ota, Z 1996, 'Apoptosis in glomerular sclerosis', Kidney International, vol. 49, no. 1, pp. 103-111.
Sugiyama, Hitoshi ; Kashihara, Naoki ; Makino, Hirofumi ; Yamasaki, Yasushi ; Ota, Zensuke. / Apoptosis in glomerular sclerosis. In: Kidney International. 1996 ; Vol. 49, No. 1. pp. 103-111.
@article{e825bf778d3942019797b776c0b1a44c,
title = "Apoptosis in glomerular sclerosis",
abstract = "Glomerulosclerosis is characterized by progressive extracellular matrix accumulation and glomerular cell loss. The role of glomerular cell apoptosis in glomerulosclerosis was investigated in the rat remnant kidney model and in human glomerular diseases. We identified apoptotic cells in the glomeruli, tubules and interstitium in the remnant kidney by electron microscopy. DNA fragmentation, which is a biochemical characteristic of apoptosis, was detected by in situ nick end-labeling of fragmented DNA with terminal deoxynucleotidyl transferase and biotinylated deoxyuridine triphosphate. Fragmented DNA in the glomeruli and tubules increased with the progression of glomerulosclerosis in the remnant kidney model. This finding was also demonstrated in other glomerular sclerotic lesions such as IgA and lupus nephritis. The number of cells positive for nick end-labeling in the glomerulus significantly correlated with the degree of glomerulosclerosis and the deterioration of renal function. These results indicate that apoptosis is, at least in part, involved in the cell deletion of various glomerular diseases leading to sclerosis.",
author = "Hitoshi Sugiyama and Naoki Kashihara and Hirofumi Makino and Yasushi Yamasaki and Zensuke Ota",
year = "1996",
language = "English",
volume = "49",
pages = "103--111",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Apoptosis in glomerular sclerosis

AU - Sugiyama, Hitoshi

AU - Kashihara, Naoki

AU - Makino, Hirofumi

AU - Yamasaki, Yasushi

AU - Ota, Zensuke

PY - 1996

Y1 - 1996

N2 - Glomerulosclerosis is characterized by progressive extracellular matrix accumulation and glomerular cell loss. The role of glomerular cell apoptosis in glomerulosclerosis was investigated in the rat remnant kidney model and in human glomerular diseases. We identified apoptotic cells in the glomeruli, tubules and interstitium in the remnant kidney by electron microscopy. DNA fragmentation, which is a biochemical characteristic of apoptosis, was detected by in situ nick end-labeling of fragmented DNA with terminal deoxynucleotidyl transferase and biotinylated deoxyuridine triphosphate. Fragmented DNA in the glomeruli and tubules increased with the progression of glomerulosclerosis in the remnant kidney model. This finding was also demonstrated in other glomerular sclerotic lesions such as IgA and lupus nephritis. The number of cells positive for nick end-labeling in the glomerulus significantly correlated with the degree of glomerulosclerosis and the deterioration of renal function. These results indicate that apoptosis is, at least in part, involved in the cell deletion of various glomerular diseases leading to sclerosis.

AB - Glomerulosclerosis is characterized by progressive extracellular matrix accumulation and glomerular cell loss. The role of glomerular cell apoptosis in glomerulosclerosis was investigated in the rat remnant kidney model and in human glomerular diseases. We identified apoptotic cells in the glomeruli, tubules and interstitium in the remnant kidney by electron microscopy. DNA fragmentation, which is a biochemical characteristic of apoptosis, was detected by in situ nick end-labeling of fragmented DNA with terminal deoxynucleotidyl transferase and biotinylated deoxyuridine triphosphate. Fragmented DNA in the glomeruli and tubules increased with the progression of glomerulosclerosis in the remnant kidney model. This finding was also demonstrated in other glomerular sclerotic lesions such as IgA and lupus nephritis. The number of cells positive for nick end-labeling in the glomerulus significantly correlated with the degree of glomerulosclerosis and the deterioration of renal function. These results indicate that apoptosis is, at least in part, involved in the cell deletion of various glomerular diseases leading to sclerosis.

UR - http://www.scopus.com/inward/record.url?scp=0030031822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030031822&partnerID=8YFLogxK

M3 - Article

C2 - 8770955

AN - SCOPUS:0030031822

VL - 49

SP - 103

EP - 111

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 1

ER -