Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation

Takehito Suzuki, Chiaki Nagamatsu, Takahiro Kushima, Ryu Miyakoshi, Kazuaki Tanaka, Hidetoshi Morita, Motoharu Sakaue, Tatsuya Takizawa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


We previously reported that nitric oxide (NO) is first detected in the uterus of a pregnant rat on gestational day 13.5 (GD13.5) and that NO levels peak on GD17.5. In addition, NO production in the uterus is mainly derived from the decidua and not the myometrium. The aim of the present study was to reveal the role of NO that peaked on GD17.5 of gestation in the decidua. To inhibit NO production, pregnant rats were continuously administered by an nitric oxide synthase inhibitor, NG-nitro-L-argininemethyl ester (L-NAME) for 48 h. In the control group, saline was infused instead of L-NAME. After treatment, the decidua were obtained from GD13.5, GD17.5 and GD21.5 rats. Apoptosis and activated caspase-3-positive cells were observed by transferase-mediated dUTP nick-end labeling (TUNEL) assay and immunohistochemistry, respectively. The caspase-3 enzyme activity was also measured in the cell lysate from the decidua. The numbers of TUNEL-positive cells and activated caspase-3-positive cells each increased and the amount of caspase-3 activity also increased significantly in rats on GD17.5 than in rats in the control group, but no changes were observed in rats on GD13.5 and GD21.5. Furthermore, enzyme activity regarding the initiator caspases, caspase-8 and -9, upstream factors for caspase-3 in the caspase cascade, was measured simultaneously on GD17.5 under the same treatment. Caspase-8 and -9 enzyme activities increased significantly in the control group; an increment of caspase-8 activity was especially prominent. The present results indicate that an inhibitor of NO production caused apoptosis through typical apoptotic signals in the decidua on GD17.5, suggesting that an NO peak in the decidua is essential to cell survival and the maintenance of uterine formation.

Original languageEnglish
Pages (from-to)455-462
Number of pages8
JournalExperimental Biology and Medicine
Issue number4
Publication statusPublished - Apr 2010
Externally publishedYes


  • Apoptosis
  • Decidua
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation'. Together they form a unique fingerprint.

Cite this