Antiviral activity of a hot water extract of black soybean against a human respiratory illness virus

Masafumi Yamai, Kazunori Tsumura, Mariko Kimura, Seiji Fukuda, Tsukasa Murakami, Yoshinobu Kimura

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Significant antiviral activity against respiratory illness viruses has been found in a hot-water extract of black soybean. This black soybean extract showed significant antiviral activity against human adenovirus type 1 and coxsackievirus B1 in a dose-dependent manner, while the hot-water extract from common yellowish soybean showed only weak activity. The antiviral activity could not be extracted from the black soybean by 70% aqueous ethanol, suggesting that saponin in the seed did not contribute to this activity. The antiviral activity was only recovered from cotyledons and not from seed coats with the hot water, showing that the activity was distributed in the cotyledons and that antocyanins in the black soybean seed coats did not contribute to the antiviral activity. The antiviral compound(s) in the black soybean was partially purified by up to 166 times by a combination of gel filtration, reversed phase HPLC, and ion-exchange HPLC. The partially purified antiviral compound showed hydrophilic and anionic properties, and a maximum absorption at 260 nm, suggesting that this antiviral fraction may contain a phenyl group(s). On the other hand, an amino acid analysis with the acid hydrolyzate and a neutral sugar analysis showed that the antiviral compound from black soybean might not be a polypeptide or glycoconjugate bearing neutral sugar(s).

Original languageEnglish
Pages (from-to)1071-1079
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number5
Publication statusPublished - May 2003

Fingerprint

Viruses
Soybeans
Antiviral Agents
Seed
soybeans
Sugars
viruses
Water
extracts
Bearings (structural)
water
Polypeptides
Amino acids
Seeds
Ion exchange
Ethanol
Gels
Cotyledon
cotyledons
Acids

Keywords

  • Antiviral activity
  • Black soybean
  • Glycine max
  • Hot-water extract
  • Respiratory illness virus

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Antiviral activity of a hot water extract of black soybean against a human respiratory illness virus. / Yamai, Masafumi; Tsumura, Kazunori; Kimura, Mariko; Fukuda, Seiji; Murakami, Tsukasa; Kimura, Yoshinobu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 5, 05.2003, p. 1071-1079.

Research output: Contribution to journalArticle

Yamai, Masafumi ; Tsumura, Kazunori ; Kimura, Mariko ; Fukuda, Seiji ; Murakami, Tsukasa ; Kimura, Yoshinobu. / Antiviral activity of a hot water extract of black soybean against a human respiratory illness virus. In: Bioscience, Biotechnology and Biochemistry. 2003 ; Vol. 67, No. 5. pp. 1071-1079.
@article{63c6777126704dd78cfe0c91aff2e11a,
title = "Antiviral activity of a hot water extract of black soybean against a human respiratory illness virus",
abstract = "Significant antiviral activity against respiratory illness viruses has been found in a hot-water extract of black soybean. This black soybean extract showed significant antiviral activity against human adenovirus type 1 and coxsackievirus B1 in a dose-dependent manner, while the hot-water extract from common yellowish soybean showed only weak activity. The antiviral activity could not be extracted from the black soybean by 70{\%} aqueous ethanol, suggesting that saponin in the seed did not contribute to this activity. The antiviral activity was only recovered from cotyledons and not from seed coats with the hot water, showing that the activity was distributed in the cotyledons and that antocyanins in the black soybean seed coats did not contribute to the antiviral activity. The antiviral compound(s) in the black soybean was partially purified by up to 166 times by a combination of gel filtration, reversed phase HPLC, and ion-exchange HPLC. The partially purified antiviral compound showed hydrophilic and anionic properties, and a maximum absorption at 260 nm, suggesting that this antiviral fraction may contain a phenyl group(s). On the other hand, an amino acid analysis with the acid hydrolyzate and a neutral sugar analysis showed that the antiviral compound from black soybean might not be a polypeptide or glycoconjugate bearing neutral sugar(s).",
keywords = "Antiviral activity, Black soybean, Glycine max, Hot-water extract, Respiratory illness virus",
author = "Masafumi Yamai and Kazunori Tsumura and Mariko Kimura and Seiji Fukuda and Tsukasa Murakami and Yoshinobu Kimura",
year = "2003",
month = "5",
language = "English",
volume = "67",
pages = "1071--1079",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "5",

}

TY - JOUR

T1 - Antiviral activity of a hot water extract of black soybean against a human respiratory illness virus

AU - Yamai, Masafumi

AU - Tsumura, Kazunori

AU - Kimura, Mariko

AU - Fukuda, Seiji

AU - Murakami, Tsukasa

AU - Kimura, Yoshinobu

PY - 2003/5

Y1 - 2003/5

N2 - Significant antiviral activity against respiratory illness viruses has been found in a hot-water extract of black soybean. This black soybean extract showed significant antiviral activity against human adenovirus type 1 and coxsackievirus B1 in a dose-dependent manner, while the hot-water extract from common yellowish soybean showed only weak activity. The antiviral activity could not be extracted from the black soybean by 70% aqueous ethanol, suggesting that saponin in the seed did not contribute to this activity. The antiviral activity was only recovered from cotyledons and not from seed coats with the hot water, showing that the activity was distributed in the cotyledons and that antocyanins in the black soybean seed coats did not contribute to the antiviral activity. The antiviral compound(s) in the black soybean was partially purified by up to 166 times by a combination of gel filtration, reversed phase HPLC, and ion-exchange HPLC. The partially purified antiviral compound showed hydrophilic and anionic properties, and a maximum absorption at 260 nm, suggesting that this antiviral fraction may contain a phenyl group(s). On the other hand, an amino acid analysis with the acid hydrolyzate and a neutral sugar analysis showed that the antiviral compound from black soybean might not be a polypeptide or glycoconjugate bearing neutral sugar(s).

AB - Significant antiviral activity against respiratory illness viruses has been found in a hot-water extract of black soybean. This black soybean extract showed significant antiviral activity against human adenovirus type 1 and coxsackievirus B1 in a dose-dependent manner, while the hot-water extract from common yellowish soybean showed only weak activity. The antiviral activity could not be extracted from the black soybean by 70% aqueous ethanol, suggesting that saponin in the seed did not contribute to this activity. The antiviral activity was only recovered from cotyledons and not from seed coats with the hot water, showing that the activity was distributed in the cotyledons and that antocyanins in the black soybean seed coats did not contribute to the antiviral activity. The antiviral compound(s) in the black soybean was partially purified by up to 166 times by a combination of gel filtration, reversed phase HPLC, and ion-exchange HPLC. The partially purified antiviral compound showed hydrophilic and anionic properties, and a maximum absorption at 260 nm, suggesting that this antiviral fraction may contain a phenyl group(s). On the other hand, an amino acid analysis with the acid hydrolyzate and a neutral sugar analysis showed that the antiviral compound from black soybean might not be a polypeptide or glycoconjugate bearing neutral sugar(s).

KW - Antiviral activity

KW - Black soybean

KW - Glycine max

KW - Hot-water extract

KW - Respiratory illness virus

UR - http://www.scopus.com/inward/record.url?scp=1542755134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542755134&partnerID=8YFLogxK

M3 - Article

C2 - 12834285

AN - SCOPUS:1542755134

VL - 67

SP - 1071

EP - 1079

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 5

ER -