TY - JOUR
T1 - Antivibrio compounds produced by Pseudomonas sp. W3
T2 - Characterisation and assessment of their safety to shrimps
AU - Rattanachuay, Pattamarat
AU - Kantachote, Duangporn
AU - Tantirungkij, Manee
AU - Nitoda, Teruhiko
AU - Kanzaki, Hiroshi
N1 - Funding Information:
Acknowledgments This work was supported by the Graduate School and Faculty of Science, Prince of Songkla University, Thailand and partly supported by Okayama University, Japan. The authors would like to thank Charoen Pokphand Group (CP), Tha Bon, Ranot, Songkhla for providing Pacific white shrimp and shrimp food. We also thank the Aquatic Animal Help Research Center and Assoc. Prof. Dr. Varaporn Vuddhakul for providing shrimp pathogens used in this study and also Dr. Brian Hodgson for useful suggestions and assistance with the English.
PY - 2011/4
Y1 - 2011/4
N2 - In order to explore compounds naturallly inhibitory to shrimp pathogenic vibrios, a culture filtrate of Pseudomonas sp. W3 at a pH of 2 was extracted with ethyl acetate (EtOAc) to produce 82.15 mg/l of a yellow-brown extract (EtOAc-W3) that had MIC values of 225-450 μg/ml against the growth of 18 shrimp pathogenic Vibrio harveyi strains. The MIC of EtOAc-W3 against the most pathogenic strain PSU 2015 was 450 μg/ml and this strain had the lowest LD50 (50% lethal dose) to pacific white shrimp (Litopenaeus vannamei, PL 21). At this MIC value, EtOAc-W3 in artificial sea water (ASW) killed strain PSU 2015; however in natural sea water, only a partial growth inhibition was observed. The toxicity to pacific white shrimp and antivibrio activity of the EtOAc-W3 were investigated by conducting an experiment with 4 sets; native control (commercial ASW), EtOAc-W3 control (MIC/10, 45 μg/ml), challenge (inoculation 6.0 × 106 c.f.u./ml PSU 2015) and treatment (6.0 × 106 c.f.u./ml PSU 2015 + 45 μg/ml EtOAc-W3). The same experiment was repeated by increasing the dose of EtOAc-W3 to 90 μg/ml (MIC/5). Both concentrations of EtOAc-W3 tested had no toxicity to postlarval shrimps. A significant decrease in shrimp mortality was observed over a 72 h period as approximately 80% of the shrimps died in each challenge set but only 63 and 23% died in the presence of 45 and 90 μg/ml EtOAc-W3. The major component of EtOAc-W3 was supposed to be 2-heptyl-4-quinolone (HHQ) by FAB-MS and 1H-NMR analyses of the purified fraction.
AB - In order to explore compounds naturallly inhibitory to shrimp pathogenic vibrios, a culture filtrate of Pseudomonas sp. W3 at a pH of 2 was extracted with ethyl acetate (EtOAc) to produce 82.15 mg/l of a yellow-brown extract (EtOAc-W3) that had MIC values of 225-450 μg/ml against the growth of 18 shrimp pathogenic Vibrio harveyi strains. The MIC of EtOAc-W3 against the most pathogenic strain PSU 2015 was 450 μg/ml and this strain had the lowest LD50 (50% lethal dose) to pacific white shrimp (Litopenaeus vannamei, PL 21). At this MIC value, EtOAc-W3 in artificial sea water (ASW) killed strain PSU 2015; however in natural sea water, only a partial growth inhibition was observed. The toxicity to pacific white shrimp and antivibrio activity of the EtOAc-W3 were investigated by conducting an experiment with 4 sets; native control (commercial ASW), EtOAc-W3 control (MIC/10, 45 μg/ml), challenge (inoculation 6.0 × 106 c.f.u./ml PSU 2015) and treatment (6.0 × 106 c.f.u./ml PSU 2015 + 45 μg/ml EtOAc-W3). The same experiment was repeated by increasing the dose of EtOAc-W3 to 90 μg/ml (MIC/5). Both concentrations of EtOAc-W3 tested had no toxicity to postlarval shrimps. A significant decrease in shrimp mortality was observed over a 72 h period as approximately 80% of the shrimps died in each challenge set but only 63 and 23% died in the presence of 45 and 90 μg/ml EtOAc-W3. The major component of EtOAc-W3 was supposed to be 2-heptyl-4-quinolone (HHQ) by FAB-MS and 1H-NMR analyses of the purified fraction.
KW - Antivibrio compounds
KW - Pseudomonas sp.
KW - Shrimp
KW - Toxicity
KW - Vibrio harveyi
UR - http://www.scopus.com/inward/record.url?scp=79952574779&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952574779&partnerID=8YFLogxK
U2 - 10.1007/s11274-010-0529-x
DO - 10.1007/s11274-010-0529-x
M3 - Article
AN - SCOPUS:79952574779
VL - 27
SP - 869
EP - 880
JO - Mircen Journal of Applied Microbiology and Biotechnology
JF - Mircen Journal of Applied Microbiology and Biotechnology
SN - 0265-0762
IS - 4
ER -