Antitumor effect of adenovirus-mediated Bax gene transfer on p53- sensitive and p53-resistant cancer lines

Shunsuke Kagawa, Jian Gu, Stephen G. Swisher, Lin Ji, Jack A. Roth, Dejian Lai, L. Clifton Stephens, Bingliang Fang

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Antitumor effects of the proapoptotic Bax gene have been evaluated in vitro and in vivo by a binary adenovirus system expressing the human Bax gene. Overexpression of the Bax gene in cultured cell lines from human lung carcinoma results in caspase activation, apoptosis induction, and cell growth suppression. Intratumoral injection of adenovirus vector expressing the Bax gene suppressed growth of human lung cancer xenografts established in nude mice. Histological examination of tumors from mice treated with the Bax gene demonstrated high levels of Bax expression and extensive apoptosis in tumors. In comparison with the treatment by an adenoviral vector expressing human p53, the Bax gene can effectively suppress tumor growth in both p53-sensitive and p53-resistant human lung carcinoma cell lines. Toxicity was not detected in liver and other systems in animals treated intralesionally with the Bax gene. Therefore, our results suggest that the Bax gene may be useful in cancer treatment.

Original languageEnglish
Pages (from-to)1157-1161
Number of pages5
JournalCancer Research
Volume60
Issue number5
Publication statusPublished - Mar 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kagawa, S., Gu, J., Swisher, S. G., Ji, L., Roth, J. A., Lai, D., Stephens, L. C., & Fang, B. (2000). Antitumor effect of adenovirus-mediated Bax gene transfer on p53- sensitive and p53-resistant cancer lines. Cancer Research, 60(5), 1157-1161.