Antisense oligonucleotides targeting c-fos mRNA inhibit rheumatoid synovial fibroblast proliferation

Yoshitaka Morita, Naoki Kashihara, Masahiro Yamamura, Hideyuki Okamoto, Seishi Harada, Masanori Kawashima, Hirofumi Makino

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21 Citations (Scopus)

Abstract

Objective - To determine whether andsense oligonucleotides targeting c- fos mRNA have the ability to inhibit the growth of interleukin 1 (IL1) stimulated fibroblast-like cells from the synovium in rheumatoid arthritis (RA). Methods - Fibroblast-like cells established from RA synovium were stimulated by IL1 with antisense or sense oligonucleotides complementary to c-fos mRNA, and the proliferation of these cells was determined by 3H- thymidine incorporation. Effect of antisense ollgonucleotides on expression of activator protein 1 (AP1) activity was evaluated using electrophoretic mobility shift assay. Results - C-fos antisense oligonucleotides inhibited IL1 stimulated synovial fibroblast proliferation. The expression of AP1 activity induced by IL1 was suppressed by treatment with antisense oligonucleotides. Conclusion - These results suggest the feasibility of antisense strategies designed to suppress c-fos expression as therapeutic agents for RA.

Original languageEnglish
Pages (from-to)122-124
Number of pages3
JournalAnnals of the Rheumatic Diseases
Volume57
Issue number2
DOIs
Publication statusPublished - Jan 1 1998

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ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

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