Antisense oligonucleotides targeting c-fos mRNA inhibit rheumatoid synovial fibroblast proliferation

Yoshitaka Morita, Naoki Kashihara, Masahiro Yamamura, Hideyuki Okamoto, Seishi Harada, Masanori Kawashima, Hirofumi Makino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective - To determine whether andsense oligonucleotides targeting c- fos mRNA have the ability to inhibit the growth of interleukin 1 (IL1) stimulated fibroblast-like cells from the synovium in rheumatoid arthritis (RA). Methods - Fibroblast-like cells established from RA synovium were stimulated by IL1 with antisense or sense oligonucleotides complementary to c-fos mRNA, and the proliferation of these cells was determined by 3H- thymidine incorporation. Effect of antisense ollgonucleotides on expression of activator protein 1 (AP1) activity was evaluated using electrophoretic mobility shift assay. Results - C-fos antisense oligonucleotides inhibited IL1 stimulated synovial fibroblast proliferation. The expression of AP1 activity induced by IL1 was suppressed by treatment with antisense oligonucleotides. Conclusion - These results suggest the feasibility of antisense strategies designed to suppress c-fos expression as therapeutic agents for RA.

Original languageEnglish
Pages (from-to)122-124
Number of pages3
JournalAnnals of the Rheumatic Diseases
Volume57
Issue number2
DOIs
Publication statusPublished - Jan 1 1998

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Antisense Oligonucleotides
Fibroblasts
Interleukin-1
Rheumatoid Arthritis
Messenger RNA
Synovial Membrane
Transcription Factor AP-1
Oligonucleotides
Electrophoretic mobility
Electrophoretic Mobility Shift Assay
Thymidine
Assays
Cell Proliferation
Growth
Therapeutics

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Antisense oligonucleotides targeting c-fos mRNA inhibit rheumatoid synovial fibroblast proliferation. / Morita, Yoshitaka; Kashihara, Naoki; Yamamura, Masahiro; Okamoto, Hideyuki; Harada, Seishi; Kawashima, Masanori; Makino, Hirofumi.

In: Annals of the Rheumatic Diseases, Vol. 57, No. 2, 01.01.1998, p. 122-124.

Research output: Contribution to journalArticle

Morita, Yoshitaka ; Kashihara, Naoki ; Yamamura, Masahiro ; Okamoto, Hideyuki ; Harada, Seishi ; Kawashima, Masanori ; Makino, Hirofumi. / Antisense oligonucleotides targeting c-fos mRNA inhibit rheumatoid synovial fibroblast proliferation. In: Annals of the Rheumatic Diseases. 1998 ; Vol. 57, No. 2. pp. 122-124.
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