Antisense oligodeoxynucleotides: useful tool for search and assessment of new targets for anti-malarial drugs.

N. Ikemoto, H. S. Kim, M. Kanazaki, Y. Ueno, S. Shuto, A. Matsuda, Y. Wataya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4'alpha-C-(2-aminoethyl)thymidines (4'-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iron-sulfur subunit (IP), had antimalarial activity (EC50; about 1.0 microM). Furthermore we showed that intra-parasitic SDH IP mRNA levels, which were detected using quantitative RT-PCR assay, were decreased 13% of control after the 24 h expose to SDH IP AS. From the results, we conclude that SDH has potential as the target for novel antimalarials, and AS ODNs is effective for search and assessment of targets for new antimalarial drugs.

Original languageEnglish
Pages (from-to)89-90
Number of pages2
JournalNucleic acids symposium series
Issue number42
DOIs
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Medicine(all)

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