Antisense oligodeoxynucleotides: useful tool for search and assessment of new targets for anti-malarial drugs.

N. Ikemoto, Hye-Sook Kim, M. Kanazaki, Y. Ueno, S. Shuto, A. Matsuda, Y. Wataya

Research output: Contribution to journalArticle

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Abstract

We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4'alpha-C-(2-aminoethyl)thymidines (4'-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iron-sulfur subunit (IP), had antimalarial activity (EC50; about 1.0 microM). Furthermore we showed that intra-parasitic SDH IP mRNA levels, which were detected using quantitative RT-PCR assay, were decreased 13% of control after the 24 h expose to SDH IP AS. From the results, we conclude that SDH has potential as the target for novel antimalarials, and AS ODNs is effective for search and assessment of targets for new antimalarial drugs.

Original languageEnglish
Pages (from-to)89-90
Number of pages2
JournalNucleic acids symposium series
Issue number42
Publication statusPublished - 1999

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Oligodeoxyribonucleotides
Antimalarials
Succinate Dehydrogenase
Pharmaceutical Preparations
Sulfur
Thymidine
Iron
Polymerase Chain Reaction
Messenger RNA

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Antisense oligodeoxynucleotides : useful tool for search and assessment of new targets for anti-malarial drugs. / Ikemoto, N.; Kim, Hye-Sook; Kanazaki, M.; Ueno, Y.; Shuto, S.; Matsuda, A.; Wataya, Y.

In: Nucleic acids symposium series, No. 42, 1999, p. 89-90.

Research output: Contribution to journalArticle

Ikemoto, N. ; Kim, Hye-Sook ; Kanazaki, M. ; Ueno, Y. ; Shuto, S. ; Matsuda, A. ; Wataya, Y. / Antisense oligodeoxynucleotides : useful tool for search and assessment of new targets for anti-malarial drugs. In: Nucleic acids symposium series. 1999 ; No. 42. pp. 89-90.
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