Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells

Toshio Noguchi, Kazuma Ikeda, Kazuhiko Yamamoto, Isao Yoshida, Atsuko Ashiba, Junjiro Tsuchiyama, Katsuji Shinagawa, Tadashi Yoshino, Minoru Takata, Mine Harada

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17 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) is essential for immortalization of B cells by EBV, protects the infected cells from apoptotic cell death and induces Bcl-2 expression. Suppression of LMP-1 expression by antisense oligodeoxynucleotides (AS-oligo) to LMP-1 inhibits proliferation, promotes apoptosis and suppresses Bcl-2 expression in EBV-transformed B cells. However, the function of LMP-1 expression in EBV-positive natural killer (NK) cell lymphoma cells has not been reported previously. We examined the function of LMP-1 in two EBV-positive NK cell lymphoma cell lines (NK-YS and YT) through suppressing LMP-1 expression by AS-oligo to LMP-1. The AS-oligo to LMP-1 suppressed LMP-1 mRNA and protein expression in two EBV-positive NK cell lymphoma cell lines, as well as in an EBV-transformed B-cell line (CMG-1). Proliferation was inhibited, apoptosis was induced and Bcl-2 expression was suppressed in CMG-1 cells, but none of these events were observed in NK-YS or YT cells. These results suggest that proliferation, inhibition of apoptosis and Bcl-2 expression in EBV-positive NK cell lymphoma cells are not directly regulated by LMP-1 as in EBV-transformed B-cell lines, but are probably mediated through other signal transducing systems.

Original languageEnglish
Pages (from-to)84-92
Number of pages9
JournalBritish Journal of Haematology
Volume114
Issue number1
DOIs
Publication statusPublished - Aug 14 2001

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Keywords

  • Antisense oligodeoxynucleotides
  • Apoptosis
  • EBV-positive NK-cell lymphoma
  • LMP-1
  • Proliferation

ASJC Scopus subject areas

  • Hematology

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