Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

Mizuki Kitamatsu, Shunsuke Kurami, Takashi Ohtsuki, Masahiko Sisido

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.

Original languageEnglish
Pages (from-to)225-227
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

Fingerprint

Peptide Nucleic Acids
Escherichia coli
Cell-Penetrating Peptides
Assays
Switches
pyrrolidine
Temperature

Keywords

  • Antisense effect
  • Cell-penetrating peptide
  • Peptide nucleic acid

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

Cite this

Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli. / Kitamatsu, Mizuki; Kurami, Shunsuke; Ohtsuki, Takashi; Sisido, Masahiko.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 21, No. 1, 01.01.2011, p. 225-227.

Research output: Contribution to journalArticle

Kitamatsu, Mizuki ; Kurami, Shunsuke ; Ohtsuki, Takashi ; Sisido, Masahiko. / Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli. In: Bioorganic and Medicinal Chemistry Letters. 2011 ; Vol. 21, No. 1. pp. 225-227.
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