Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

Mizuki Kitamatsu, Shunsuke Kurami, Takashi Ohtsuki, Masahiko Sisido

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.

Original languageEnglish
Pages (from-to)225-227
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

Keywords

  • Antisense effect
  • Cell-penetrating peptide
  • Peptide nucleic acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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