Antiscorbutic Activity of L-Ascorbic Acid 2-Glucoside and Its Availability as a Vitamin C Supplement in Normal Rats and Guinea Pigs

Itaru Yamamoto, Sadaharu Suga, Yoshihiro Mitoh, Mari Tanaka, Norio Muto

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Bioavailability of a newly-synthesized and chemically-stable 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) as a vitamin C supplement was investigated in rats and guinea pigs. Oral administration of AA-2G to the animals resulted in an increase of serum ascorbic acid (AA) levels. However, in these sera the intact form was not detectable by the high performance liquid chromatography (HPLC) method, indicating its hydrolysis through the process of absorption. After an intravenous injection of AA-2G, the intact form diminished rapidly from the serum, followed by prolonged and marked elevation of serum AA levels. Various tissue homogenates from rats and guinea pigs were examined for their releasing activity of AA from AA-2G. High activity was observed in kidney, small intestine and serum of rats and in small intestine and kidney of guinea pigs. These hydrolytic activities were completely inhibited by castanospermine, a specific a-glucosidase inhibitor, suggesting the participation of a-glucosidase in the in vivo hydrolysis of AA-2G. AA-2G was found to exhibit obvious therapeutic effect in scorbutic guinea pigs by its repeated oral administrations. These results indicate that AA-2G is a readily available source of vitamin C activity in vivo.

Original languageEnglish
Pages (from-to)688-695
Number of pages8
Journaljournal of pharmacobio-dynamics
Volume13
Issue number11
DOIs
Publication statusPublished - Jan 1 1990

Keywords

  • a-glucosidase
  • antiscorbutic activity
  • ascorbic acid
  • ascorbic acid 2-glucoside
  • bioavailability
  • in vivo hydrolysis
  • stable ascorbic acid
  • vitamin C supplement

ASJC Scopus subject areas

  • Pharmacology

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