Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas

Toshiya Tsuji, Isao Nozaki, Masahiro Miyazaki, Masakiyo Sakaguchi, Hong Pu, Yusuke Hamazaki, Osamu Iijima, Masayoshi Namba

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

We recently reported the cloning of the REIC/Dkk-3 gene, whose expression was shown to be down-regulated in many human immortalized and tumor-derived cell lines [T. Tsuji et al. (2000) Biochem. Biophys. Res. Commun. 268, 20-24]. In the present study, we demonstrated that expression of the exogenous REIC/Dkk-3 gene in tumor cells inhibited cell growth. Furthermore, the level of REIC/Dkk-3 mRNA in normal human cells was lowest in the late G1 phase during the cell cycle. Then we found that the expression of REIC/Dkk-3 was significantly down-regulated in surgically resected non-small-cell lung carcinomas. We determined the REIC/Dkk-3 locus on chromosome 11p15, where loss of heterozygosity has frequently been observed in human tumors. These findings indicate that REIC/Dkk-3 may function as a tumor suppressor.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number1
DOIs
Publication statusPublished - Nov 23 2001

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Non-Small Cell Lung Carcinoma
Down-Regulation
Cells
Tumors
Neoplasms
Loss of Heterozygosity
G1 Phase
Tumor Cell Line
Organism Cloning
Cell Cycle
Chromosomes
Cloning
Gene Expression
Cell growth
Messenger RNA
Gene expression
Growth
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas. / Tsuji, Toshiya; Nozaki, Isao; Miyazaki, Masahiro; Sakaguchi, Masakiyo; Pu, Hong; Hamazaki, Yusuke; Iijima, Osamu; Namba, Masayoshi.

In: Biochemical and Biophysical Research Communications, Vol. 289, No. 1, 23.11.2001, p. 257-263.

Research output: Contribution to journalArticle

Tsuji, Toshiya ; Nozaki, Isao ; Miyazaki, Masahiro ; Sakaguchi, Masakiyo ; Pu, Hong ; Hamazaki, Yusuke ; Iijima, Osamu ; Namba, Masayoshi. / Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 289, No. 1. pp. 257-263.
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