Antioxidant Defense Mechanisms of Salinity Tolerance in Rice Genotypes

Mohammad Golam Kibria, Mahmud Hossain, Yoshiyuki Murata, Md Anamul Hoque

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In order to elucidate the role of antioxidant responses in salinity tolerance in rice genotypes under salt stress, experiments were conducted using four rice varieties, including salt-sensitive BRRI dhan 28 and three salt-tolerant varieties BRRI dhan 47, BINA dhan 8 and BINA dhan 10. Thirty-day-old rice seedlings were transplanted into pots. At the active tillering stage (35 d after transplanting), plants were exposed to different salinity levels (0, 20, 40 and 60 mmol/L NaCl). Salt stress caused a significant reduction in growth for all the rice genotypes. Growth reduction was higher in the salt-sensitive genotype than in the salt-tolerant ones, and BINA dhan 10 showed higher salt tolerance in all measured physiological parameters. The reduction in shoot and root biomass was found to be minimal in BINA dhan 10. Chlorophyll content significantly decreased under salt stress except for BINA dhan 10. Proline content significantly increased in salt-tolerant rice genotypes with increased salt concentration, and the highest proline content was obtained from BINA dhan 10 under salt stress. Catalase and ascorbate peroxidase activities significantly decreased in salt-sensitive genotype whereas significantly increased in salt-tolerant ones with increasing salt concentration. However, salt stress significantly decreased guaiacol peroxidase activity in all the rice genotypes irrespective of salt tolerance. K+/Na+ ratio also significantly decreased in shoots and roots of all the rice genotypes. The salt-tolerant genotype BINA dhan 10 maintained higher levels of chlorophyll and proline contents as well as catalase and ascorbate peroxidase activities under salt stress, thus, this might be the underlying mechanism for salt tolerance.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalRice Science
Volume24
Issue number3
DOIs
Publication statusPublished - May 1 2017

Fingerprint

Salinity
defense mechanisms
Salts
Antioxidants
antioxidant activity
Genotype
salinity
salts
salt stress
rice
genotype
salt tolerance
proline
ascorbate peroxidase
Salt-Tolerance
salt concentration
growth retardation
catalase
Proline
Ascorbate Peroxidases

Keywords

  • antioxidant enzyme
  • chlorophyll content
  • K/Na ratio
  • proline content
  • rice
  • salinity

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Antioxidant Defense Mechanisms of Salinity Tolerance in Rice Genotypes. / Kibria, Mohammad Golam; Hossain, Mahmud; Murata, Yoshiyuki; Hoque, Md Anamul.

In: Rice Science, Vol. 24, No. 3, 01.05.2017, p. 155-162.

Research output: Contribution to journalArticle

Kibria, Mohammad Golam ; Hossain, Mahmud ; Murata, Yoshiyuki ; Hoque, Md Anamul. / Antioxidant Defense Mechanisms of Salinity Tolerance in Rice Genotypes. In: Rice Science. 2017 ; Vol. 24, No. 3. pp. 155-162.
@article{6dfc8c8efb3543b9bb01d357ba4cbb70,
title = "Antioxidant Defense Mechanisms of Salinity Tolerance in Rice Genotypes",
abstract = "In order to elucidate the role of antioxidant responses in salinity tolerance in rice genotypes under salt stress, experiments were conducted using four rice varieties, including salt-sensitive BRRI dhan 28 and three salt-tolerant varieties BRRI dhan 47, BINA dhan 8 and BINA dhan 10. Thirty-day-old rice seedlings were transplanted into pots. At the active tillering stage (35 d after transplanting), plants were exposed to different salinity levels (0, 20, 40 and 60 mmol/L NaCl). Salt stress caused a significant reduction in growth for all the rice genotypes. Growth reduction was higher in the salt-sensitive genotype than in the salt-tolerant ones, and BINA dhan 10 showed higher salt tolerance in all measured physiological parameters. The reduction in shoot and root biomass was found to be minimal in BINA dhan 10. Chlorophyll content significantly decreased under salt stress except for BINA dhan 10. Proline content significantly increased in salt-tolerant rice genotypes with increased salt concentration, and the highest proline content was obtained from BINA dhan 10 under salt stress. Catalase and ascorbate peroxidase activities significantly decreased in salt-sensitive genotype whereas significantly increased in salt-tolerant ones with increasing salt concentration. However, salt stress significantly decreased guaiacol peroxidase activity in all the rice genotypes irrespective of salt tolerance. K+/Na+ ratio also significantly decreased in shoots and roots of all the rice genotypes. The salt-tolerant genotype BINA dhan 10 maintained higher levels of chlorophyll and proline contents as well as catalase and ascorbate peroxidase activities under salt stress, thus, this might be the underlying mechanism for salt tolerance.",
keywords = "antioxidant enzyme, chlorophyll content, K/Na ratio, proline content, rice, salinity",
author = "Kibria, {Mohammad Golam} and Mahmud Hossain and Yoshiyuki Murata and Hoque, {Md Anamul}",
year = "2017",
month = "5",
day = "1",
doi = "10.1016/j.rsci.2017.05.001",
language = "English",
volume = "24",
pages = "155--162",
journal = "Rice Science",
issn = "1672-6308",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Antioxidant Defense Mechanisms of Salinity Tolerance in Rice Genotypes

AU - Kibria, Mohammad Golam

AU - Hossain, Mahmud

AU - Murata, Yoshiyuki

AU - Hoque, Md Anamul

PY - 2017/5/1

Y1 - 2017/5/1

N2 - In order to elucidate the role of antioxidant responses in salinity tolerance in rice genotypes under salt stress, experiments were conducted using four rice varieties, including salt-sensitive BRRI dhan 28 and three salt-tolerant varieties BRRI dhan 47, BINA dhan 8 and BINA dhan 10. Thirty-day-old rice seedlings were transplanted into pots. At the active tillering stage (35 d after transplanting), plants were exposed to different salinity levels (0, 20, 40 and 60 mmol/L NaCl). Salt stress caused a significant reduction in growth for all the rice genotypes. Growth reduction was higher in the salt-sensitive genotype than in the salt-tolerant ones, and BINA dhan 10 showed higher salt tolerance in all measured physiological parameters. The reduction in shoot and root biomass was found to be minimal in BINA dhan 10. Chlorophyll content significantly decreased under salt stress except for BINA dhan 10. Proline content significantly increased in salt-tolerant rice genotypes with increased salt concentration, and the highest proline content was obtained from BINA dhan 10 under salt stress. Catalase and ascorbate peroxidase activities significantly decreased in salt-sensitive genotype whereas significantly increased in salt-tolerant ones with increasing salt concentration. However, salt stress significantly decreased guaiacol peroxidase activity in all the rice genotypes irrespective of salt tolerance. K+/Na+ ratio also significantly decreased in shoots and roots of all the rice genotypes. The salt-tolerant genotype BINA dhan 10 maintained higher levels of chlorophyll and proline contents as well as catalase and ascorbate peroxidase activities under salt stress, thus, this might be the underlying mechanism for salt tolerance.

AB - In order to elucidate the role of antioxidant responses in salinity tolerance in rice genotypes under salt stress, experiments were conducted using four rice varieties, including salt-sensitive BRRI dhan 28 and three salt-tolerant varieties BRRI dhan 47, BINA dhan 8 and BINA dhan 10. Thirty-day-old rice seedlings were transplanted into pots. At the active tillering stage (35 d after transplanting), plants were exposed to different salinity levels (0, 20, 40 and 60 mmol/L NaCl). Salt stress caused a significant reduction in growth for all the rice genotypes. Growth reduction was higher in the salt-sensitive genotype than in the salt-tolerant ones, and BINA dhan 10 showed higher salt tolerance in all measured physiological parameters. The reduction in shoot and root biomass was found to be minimal in BINA dhan 10. Chlorophyll content significantly decreased under salt stress except for BINA dhan 10. Proline content significantly increased in salt-tolerant rice genotypes with increased salt concentration, and the highest proline content was obtained from BINA dhan 10 under salt stress. Catalase and ascorbate peroxidase activities significantly decreased in salt-sensitive genotype whereas significantly increased in salt-tolerant ones with increasing salt concentration. However, salt stress significantly decreased guaiacol peroxidase activity in all the rice genotypes irrespective of salt tolerance. K+/Na+ ratio also significantly decreased in shoots and roots of all the rice genotypes. The salt-tolerant genotype BINA dhan 10 maintained higher levels of chlorophyll and proline contents as well as catalase and ascorbate peroxidase activities under salt stress, thus, this might be the underlying mechanism for salt tolerance.

KW - antioxidant enzyme

KW - chlorophyll content

KW - K/Na ratio

KW - proline content

KW - rice

KW - salinity

UR - http://www.scopus.com/inward/record.url?scp=85019093318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019093318&partnerID=8YFLogxK

U2 - 10.1016/j.rsci.2017.05.001

DO - 10.1016/j.rsci.2017.05.001

M3 - Article

AN - SCOPUS:85019093318

VL - 24

SP - 155

EP - 162

JO - Rice Science

JF - Rice Science

SN - 1672-6308

IS - 3

ER -