Antioxidant capacities in various animal sera as measured with multiple free-radical scavenging method

Yoshimi Sueishi, Risako Nii, Chikako Uda, A. Takashima

Research output: Contribution to journalArticlepeer-review

Abstract

Scavenging abilities of animal sera against six reactive species (OH[rad], O2 −[rad], RO[rad], t-BuOO[rad], H3C[rad], and 1O2) were determined with the use of multiple free-radical scavenging (MULTIS) method. Commercially available sera from pig, horse, rabbit, Guinea pig, hamster and chicken were subjected to MULTIS analysis and the results were compared with human specimen. In general, animal sera showed lower scavenging ability against OH[rad] and RO[rad] radicals than human serum. However, it is noteworthy that rabbit and chicken sera have higher scavenging ability against O2 −[rad] than others. This is consistent with the known data that superoxide dismutase levels in these sera are high. In addition, we determined the uric acid level in animal sera using the uricase-TOOS method. In chicken serum, uric acid was found to be the major effective component in RO[rad] scavenging. This paper is first to quantitatively evaluate antioxidant capacities in animal sera.

Original languageEnglish
Pages (from-to)2145-2149
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume29
Issue number16
DOIs
Publication statusPublished - Aug 15 2019

Keywords

  • Animal serum
  • Antioxidant ability
  • ESR spin-trapping
  • Multiple radical scavenging
  • Uric acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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