TY - JOUR
T1 - Antioxidant capacities in various animal sera as measured with multiple free-radical scavenging method
AU - Sueishi, Yoshimi
AU - Nii, Risako
AU - Uda, Chikako
AU - Takashima, A.
N1 - Funding Information:
We thank Dr. Yashige Kotake for helpful discussion and critical reading of the manuscript. This work was supported by a research grant from SBI Pharmaceuticals Co., Ltd. , Japan.
Funding Information:
We thank Dr. Yashige Kotake for helpful discussion and critical reading of the manuscript. This work was supported by a research grant from SBI Pharmaceuticals Co. Ltd., Japan. The authors declare no conflicts of interest. This article does not contain any studies with human participants of animals performed by any of the authors.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/8/15
Y1 - 2019/8/15
N2 - Scavenging abilities of animal sera against six reactive species (OH[rad], O2 −[rad], RO[rad], t-BuOO[rad], H3C[rad], and 1O2) were determined with the use of multiple free-radical scavenging (MULTIS) method. Commercially available sera from pig, horse, rabbit, Guinea pig, hamster and chicken were subjected to MULTIS analysis and the results were compared with human specimen. In general, animal sera showed lower scavenging ability against OH[rad] and RO[rad] radicals than human serum. However, it is noteworthy that rabbit and chicken sera have higher scavenging ability against O2 −[rad] than others. This is consistent with the known data that superoxide dismutase levels in these sera are high. In addition, we determined the uric acid level in animal sera using the uricase-TOOS method. In chicken serum, uric acid was found to be the major effective component in RO[rad] scavenging. This paper is first to quantitatively evaluate antioxidant capacities in animal sera.
AB - Scavenging abilities of animal sera against six reactive species (OH[rad], O2 −[rad], RO[rad], t-BuOO[rad], H3C[rad], and 1O2) were determined with the use of multiple free-radical scavenging (MULTIS) method. Commercially available sera from pig, horse, rabbit, Guinea pig, hamster and chicken were subjected to MULTIS analysis and the results were compared with human specimen. In general, animal sera showed lower scavenging ability against OH[rad] and RO[rad] radicals than human serum. However, it is noteworthy that rabbit and chicken sera have higher scavenging ability against O2 −[rad] than others. This is consistent with the known data that superoxide dismutase levels in these sera are high. In addition, we determined the uric acid level in animal sera using the uricase-TOOS method. In chicken serum, uric acid was found to be the major effective component in RO[rad] scavenging. This paper is first to quantitatively evaluate antioxidant capacities in animal sera.
KW - Animal serum
KW - Antioxidant ability
KW - ESR spin-trapping
KW - Multiple radical scavenging
KW - Uric acid
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U2 - 10.1016/j.bmcl.2019.06.057
DO - 10.1016/j.bmcl.2019.06.057
M3 - Article
C2 - 31272792
AN - SCOPUS:85068037424
SN - 0960-894X
VL - 29
SP - 2145
EP - 2149
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 16
ER -