Antineuroinflammatory Effect of SMTP-7 in Ischemic Mice

Yong Huang, Yasuyuki Ohta, Jingwei Shang, Ryuta Morihara, Yumiko Nakano, Yusuke Fukui, Xia Liu, Xiaowen Shi, Tian Feng, Toru Yamashita, Kota Sato, Mami Takemoto, Nozomi Hishikawa, Eriko Suzuki, Keiji Hasumi, Koji Abe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Stachybotrys microspora triprenyl phenol-7 (SMTP-7) has both potentials of thrombolytic and neuroprotective effects, but its detailed neuroprotective mechanisms in ischemic stroke are still unclear. Here, we assessed the neuroprotective effects of SMTP-7 for anti-inflammatory and antiapoptosis mechanisms after 60 minutes of transient middle cerebral artery occlusion (tMCAO) in mice. Methods: After 60 minutes of tMCAO, 0.9% NaCl, tissue-type plasminogen activator (tPA), SMTP-7 or tPA+SMTP-7 was intravenously administrated through subclavian vein just before the reperfusion, and these mice were examined at 24 hours after reperfusion. We histologically assessed the antineuroinflammatory effect of SMTP-7 on the expressive changes of inflammatory markers in ischemic mouse brains. Results: Compared with the vehicle and tPA groups, SMTP-7 treatment significantly improved clinical scores and decreased the infarct volume and the numbers of TNF-α, nuclear factor-κB (NF-κB), nucleotide oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3), and cleaved caspase-3-positive cells in the brain of mice at 24 hours after tMCAO but not p62-positive cells. However, tPA+SMTP-7 treatment did not show such effects. Conclusions: The present study suggested that SMTP-7 provides a therapeutic benefit for ischemic stroke mice through anti-inflammatory and antiapoptotic effects but not antiautophagic effect.

Original languageEnglish
Pages (from-to)3084-3094
Number of pages11
JournalJournal of Stroke and Cerebrovascular Diseases
Volume27
Issue number11
DOIs
Publication statusPublished - Nov 2018

Keywords

  • Apoptosis—Inflammation—Ischemic stroke—tPA—SMTP-7

ASJC Scopus subject areas

  • Surgery
  • Rehabilitation
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Huang, Y., Ohta, Y., Shang, J., Morihara, R., Nakano, Y., Fukui, Y., Liu, X., Shi, X., Feng, T., Yamashita, T., Sato, K., Takemoto, M., Hishikawa, N., Suzuki, E., Hasumi, K., & Abe, K. (2018). Antineuroinflammatory Effect of SMTP-7 in Ischemic Mice. Journal of Stroke and Cerebrovascular Diseases, 27(11), 3084-3094. https://doi.org/10.1016/j.jstrokecerebrovasdis.2018.06.039