Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts

Yoshichika Taira; Yuki Okegawa; Kazuhiko Sugimoto; Masato Abe; Hideto Miyoshi; Toshiharu Shikanai

doi:10.1016/j.fob.2013.09.007

Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts

Yoshichika Taira, Yuki Okegawa, Kazuhiko Sugimoto, Masato Abe, Hideto Miyoshi, Toshiharu Shikanai

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Antimycin A₃ (AA) is used as an inhibitor of cyclic electron transport around photosystem I. However, the high concentrations of AA that are needed for inhibition have secondary effects, even in chloroplasts. Here, we screened for chemicals that inhibited ferredoxin-dependent plastoquinone reduction in ruptured chloroplasts at lower concentrations than those required for AA. We identified two AA-like compounds: AAL1 and AAL2. AAL1 likely shares an inhibitory site with AA, most probably in the PGR5-PGRL1 protein complex, and enhances O₂ evolution in photosystem II, most likely via an uncoupler-like effect. AAL1 and AAL2 are unlikely to penetrate intact leaves. In ruptured chloroplasts, AALs are superior to AA as inhibitors of cyclic electron transport.

Original language	English
Pages (from-to)	406-410
Number of pages	5
Journal	FEBS Open Bio
Volume	3
DOIs	https://doi.org/10.1016/j.fob.2013.09.007
Publication status	Published - 2013
Externally published	Yes

Keywords

Antimycin A
Chloroplast
Cyclic electron transport
PGR5
Photosynthesis

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1016/j.fob.2013.09.007

Cite this

@article{bfd8f6731d884951ba0da3275c7e2d64,

title = "Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts",

abstract = "Antimycin A3 (AA) is used as an inhibitor of cyclic electron transport around photosystem I. However, the high concentrations of AA that are needed for inhibition have secondary effects, even in chloroplasts. Here, we screened for chemicals that inhibited ferredoxin-dependent plastoquinone reduction in ruptured chloroplasts at lower concentrations than those required for AA. We identified two AA-like compounds: AAL1 and AAL2. AAL1 likely shares an inhibitory site with AA, most probably in the PGR5-PGRL1 protein complex, and enhances O2 evolution in photosystem II, most likely via an uncoupler-like effect. AAL1 and AAL2 are unlikely to penetrate intact leaves. In ruptured chloroplasts, AALs are superior to AA as inhibitors of cyclic electron transport.",

keywords = "Antimycin A, Chloroplast, Cyclic electron transport, PGR5, Photosynthesis",

author = "Yoshichika Taira and Yuki Okegawa and Kazuhiko Sugimoto and Masato Abe and Hideto Miyoshi and Toshiharu Shikanai",

note = "Funding Information: The authors thank Tsuyoshi Endo and Kentaro Ifuku for their support in oxygen evolution analysis. T.S. was supported by Grants 22114509 and 22247005 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. ",

year = "2013",

doi = "10.1016/j.fob.2013.09.007",

language = "English",

volume = "3",

pages = "406--410",

journal = "FEBS Open Bio",

issn = "2211-5463",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts

AU - Taira, Yoshichika

AU - Okegawa, Yuki

AU - Sugimoto, Kazuhiko

AU - Abe, Masato

AU - Miyoshi, Hideto

AU - Shikanai, Toshiharu

N1 - Funding Information: The authors thank Tsuyoshi Endo and Kentaro Ifuku for their support in oxygen evolution analysis. T.S. was supported by Grants 22114509 and 22247005 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

PY - 2013

Y1 - 2013

N2 - Antimycin A3 (AA) is used as an inhibitor of cyclic electron transport around photosystem I. However, the high concentrations of AA that are needed for inhibition have secondary effects, even in chloroplasts. Here, we screened for chemicals that inhibited ferredoxin-dependent plastoquinone reduction in ruptured chloroplasts at lower concentrations than those required for AA. We identified two AA-like compounds: AAL1 and AAL2. AAL1 likely shares an inhibitory site with AA, most probably in the PGR5-PGRL1 protein complex, and enhances O2 evolution in photosystem II, most likely via an uncoupler-like effect. AAL1 and AAL2 are unlikely to penetrate intact leaves. In ruptured chloroplasts, AALs are superior to AA as inhibitors of cyclic electron transport.

AB - Antimycin A3 (AA) is used as an inhibitor of cyclic electron transport around photosystem I. However, the high concentrations of AA that are needed for inhibition have secondary effects, even in chloroplasts. Here, we screened for chemicals that inhibited ferredoxin-dependent plastoquinone reduction in ruptured chloroplasts at lower concentrations than those required for AA. We identified two AA-like compounds: AAL1 and AAL2. AAL1 likely shares an inhibitory site with AA, most probably in the PGR5-PGRL1 protein complex, and enhances O2 evolution in photosystem II, most likely via an uncoupler-like effect. AAL1 and AAL2 are unlikely to penetrate intact leaves. In ruptured chloroplasts, AALs are superior to AA as inhibitors of cyclic electron transport.

KW - Antimycin A

KW - Chloroplast

KW - Cyclic electron transport

KW - PGR5

KW - Photosynthesis

UR - http://www.scopus.com/inward/record.url?scp=84885043919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885043919&partnerID=8YFLogxK

U2 - 10.1016/j.fob.2013.09.007

DO - 10.1016/j.fob.2013.09.007

M3 - Article

AN - SCOPUS:84885043919

SN - 2211-5463

VL - 3

SP - 406

EP - 410

JO - FEBS Open Bio

JF - FEBS Open Bio

ER -

Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this