Antimicrobial susceptibility of clinical isolates of aerobic Gram-positive cocci and anaerobic bacteria in 2008

Isamu Yoshida, Takahiro Yamaguchi, Reiko Kudo, Rieko Fuji, Choichiro Takahashi, Reiko Oota, Mitsuo Kaku, Hiroyuki Kunishima, Masahiko Okada, Yoshinori Horikawa, Joji Shiotani, Hiroyoshi Kino, Yuka Ono, Shinichi Fujita, Shuji Matsuo, Hisashi Kono, Seishi Asari, Masahiro Toyokawa, Nobuchika Kusano, Motoko NoseToshinobu Horii, Ayako Tanimoto, Hitoshi Miyamoto, Tetsunori Saikawa, Kazufumi Hiramatsu, Shigeru Kohno, Katsunori Yanagihara, Nobuhisa Yamane, Isamu Nakasone, Hideki Maki, Yoshinori Yamano

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

The activity of antibacterial agents against aerobic Gram-positive cocci (25 genus or species, 1029 strains) and anaerobic bacteria (21 genus or species, 187 strains) isolated from clinical specimens in 2008 at 16 clinical facilities in Japan were studied using either broth microdilution or agar dilution method. The ratio of methicillin-resistant strains among Staphylococcus aureus and Staphylococcus epidermidis was 59.6% and 81.2%, suggesting that resistant strains were isolated at high frequency. Vancomycin (VCM), linezolid (LZD) and quinupristin/dalfopristin (QPR/DPR) had good antibacterial activity against methicillin-resistant S. aureus and methicillin-resistant S. epidermidis, with MIC 90s of ≤2μg/mL. The ratio of penicillin (PC) intermediate and resistant strains classified by mutations of PC-binding proteins among Streptococcus pneumoniae was 92.0% that was highest among our previous reports. Cefpirome, carbapenems, VCM, teicoplanin (TEIC), LZD and QPR/DPR had MIC 90s of ≤1μg/mL against PC-intermediate and resistant S. pneumoniae strains. Against all strains of Enterococcus faecalis and Enterococcus faecium, the MICs of VCM and TEIC were under 2μg/mL, and no resistant strain was detected, suggesting that these agents had excellent activities against these species. 15.9% of E. faecalis strains and 1.2% of E. faecium strains showed intermediate to LZD. 17.1% of E. faecium strains showed intermediate or resistant to QPR/DPR. Against all strains of Clostridium difficile, the MIC of VCM was under 1μg/mL, suggesting that VCM had excellent activity. Carbapenems showed good activity against Clostridiales, Bacteroides spp., and Prevotella spp., but one strain of Bacteroides fragilis showed resistant to carbapenems. And so, the susceptibility of this species should be well-focused in the future at detecting continuously.

Original languageEnglish
Pages (from-to)49-72
Number of pages24
JournalJapanese Journal of Antibiotics
Volume65
Issue number1
Publication statusPublished - Feb 1 2012

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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    Yoshida, I., Yamaguchi, T., Kudo, R., Fuji, R., Takahashi, C., Oota, R., Kaku, M., Kunishima, H., Okada, M., Horikawa, Y., Shiotani, J., Kino, H., Ono, Y., Fujita, S., Matsuo, S., Kono, H., Asari, S., Toyokawa, M., Kusano, N., ... Yamano, Y. (2012). Antimicrobial susceptibility of clinical isolates of aerobic Gram-positive cocci and anaerobic bacteria in 2008. Japanese Journal of Antibiotics, 65(1), 49-72.