Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009): Nationwide survey of community-acquired and nosocomial MRSA

Katsunori Yanagihara, Nobuko Araki, Shinichi Watanabe, Takahiro Kinebuchi, Mitsuo Kaku, Shigefumi Maesaki, Keizo Yamaguchi, Tetsuya Matsumoto, Hiroshige Mikamo, Yoshio Takesue, Jun Ichi Kadota, Jiro Fujita, Keiji Iwatsuki, Haruko Hino, Takehiko Kaneko, Kenji Asagoe, Masami Ikeda, Akira Yasuoka, Shigeru Kohno

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, including community-acquired MRSA (CA-MRSA), in Japan. A total of 857 MRSA clinical isolates were collected from the 16 institutions throughout Japan that participated in the survey (2008-2009). The drug susceptibility and staphylococcal cassette chromosome mec (SCC. mec) typing and the presence of specific pathogenic genes were evaluated. The isolates comprised SCC. mec type II (73.6%), type IV (20%), and type I (6%). The percentage of SCC. mec type IV isolates was significantly higher in outpatients than in inpatients. Most of the isolated strains were sensitive to vancomycin (VCM, MIC ≤2 μg/mL), linezolid (MIC ≤4 μg/mL), and teicoplanin (MIC ≤8 μg/mL). Although most strains were sensitive to VCM, the MIC value of VCM for SCC. mec type II strains was higher than that for SCC. mec type IV strains. Only 4 (2.3%) of 171 SCC. mec type IV strains were Panton-Valentine leukocidin (lukS/F-PV)-positive. Thus, this result indicates a unique feature of SCC. mec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume72
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Community Hospital
Methicillin-Resistant Staphylococcus aureus
Japan
Linezolid
Teicoplanin
Vancomycin
Inpatients
Outpatients
Public Health
Clone Cells
Chromosomes
Surveys and Questionnaires
Pharmaceutical Preparations
Genes
Panton-Valentine leukocidin

Keywords

  • Antimicrobial susceptibility
  • CA-MRSA
  • Molecular characteristic
  • MRSA
  • Nationwide survey

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009) : Nationwide survey of community-acquired and nosocomial MRSA. / Yanagihara, Katsunori; Araki, Nobuko; Watanabe, Shinichi; Kinebuchi, Takahiro; Kaku, Mitsuo; Maesaki, Shigefumi; Yamaguchi, Keizo; Matsumoto, Tetsuya; Mikamo, Hiroshige; Takesue, Yoshio; Kadota, Jun Ichi; Fujita, Jiro; Iwatsuki, Keiji; Hino, Haruko; Kaneko, Takehiko; Asagoe, Kenji; Ikeda, Masami; Yasuoka, Akira; Kohno, Shigeru.

In: Diagnostic Microbiology and Infectious Disease, Vol. 72, No. 3, 03.2012, p. 253-257.

Research output: Contribution to journalArticle

Yanagihara, K, Araki, N, Watanabe, S, Kinebuchi, T, Kaku, M, Maesaki, S, Yamaguchi, K, Matsumoto, T, Mikamo, H, Takesue, Y, Kadota, JI, Fujita, J, Iwatsuki, K, Hino, H, Kaneko, T, Asagoe, K, Ikeda, M, Yasuoka, A & Kohno, S 2012, 'Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009): Nationwide survey of community-acquired and nosocomial MRSA', Diagnostic Microbiology and Infectious Disease, vol. 72, no. 3, pp. 253-257. https://doi.org/10.1016/j.diagmicrobio.2011.11.010
Yanagihara, Katsunori ; Araki, Nobuko ; Watanabe, Shinichi ; Kinebuchi, Takahiro ; Kaku, Mitsuo ; Maesaki, Shigefumi ; Yamaguchi, Keizo ; Matsumoto, Tetsuya ; Mikamo, Hiroshige ; Takesue, Yoshio ; Kadota, Jun Ichi ; Fujita, Jiro ; Iwatsuki, Keiji ; Hino, Haruko ; Kaneko, Takehiko ; Asagoe, Kenji ; Ikeda, Masami ; Yasuoka, Akira ; Kohno, Shigeru. / Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009) : Nationwide survey of community-acquired and nosocomial MRSA. In: Diagnostic Microbiology and Infectious Disease. 2012 ; Vol. 72, No. 3. pp. 253-257.
@article{b03be7a0335046f9b5b7e61a0358511a,
title = "Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009): Nationwide survey of community-acquired and nosocomial MRSA",
abstract = "This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, including community-acquired MRSA (CA-MRSA), in Japan. A total of 857 MRSA clinical isolates were collected from the 16 institutions throughout Japan that participated in the survey (2008-2009). The drug susceptibility and staphylococcal cassette chromosome mec (SCC. mec) typing and the presence of specific pathogenic genes were evaluated. The isolates comprised SCC. mec type II (73.6{\%}), type IV (20{\%}), and type I (6{\%}). The percentage of SCC. mec type IV isolates was significantly higher in outpatients than in inpatients. Most of the isolated strains were sensitive to vancomycin (VCM, MIC ≤2 μg/mL), linezolid (MIC ≤4 μg/mL), and teicoplanin (MIC ≤8 μg/mL). Although most strains were sensitive to VCM, the MIC value of VCM for SCC. mec type II strains was higher than that for SCC. mec type IV strains. Only 4 (2.3{\%}) of 171 SCC. mec type IV strains were Panton-Valentine leukocidin (lukS/F-PV)-positive. Thus, this result indicates a unique feature of SCC. mec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA.",
keywords = "Antimicrobial susceptibility, CA-MRSA, Molecular characteristic, MRSA, Nationwide survey",
author = "Katsunori Yanagihara and Nobuko Araki and Shinichi Watanabe and Takahiro Kinebuchi and Mitsuo Kaku and Shigefumi Maesaki and Keizo Yamaguchi and Tetsuya Matsumoto and Hiroshige Mikamo and Yoshio Takesue and Kadota, {Jun Ichi} and Jiro Fujita and Keiji Iwatsuki and Haruko Hino and Takehiko Kaneko and Kenji Asagoe and Masami Ikeda and Akira Yasuoka and Shigeru Kohno",
year = "2012",
month = "3",
doi = "10.1016/j.diagmicrobio.2011.11.010",
language = "English",
volume = "72",
pages = "253--257",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009)

T2 - Nationwide survey of community-acquired and nosocomial MRSA

AU - Yanagihara, Katsunori

AU - Araki, Nobuko

AU - Watanabe, Shinichi

AU - Kinebuchi, Takahiro

AU - Kaku, Mitsuo

AU - Maesaki, Shigefumi

AU - Yamaguchi, Keizo

AU - Matsumoto, Tetsuya

AU - Mikamo, Hiroshige

AU - Takesue, Yoshio

AU - Kadota, Jun Ichi

AU - Fujita, Jiro

AU - Iwatsuki, Keiji

AU - Hino, Haruko

AU - Kaneko, Takehiko

AU - Asagoe, Kenji

AU - Ikeda, Masami

AU - Yasuoka, Akira

AU - Kohno, Shigeru

PY - 2012/3

Y1 - 2012/3

N2 - This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, including community-acquired MRSA (CA-MRSA), in Japan. A total of 857 MRSA clinical isolates were collected from the 16 institutions throughout Japan that participated in the survey (2008-2009). The drug susceptibility and staphylococcal cassette chromosome mec (SCC. mec) typing and the presence of specific pathogenic genes were evaluated. The isolates comprised SCC. mec type II (73.6%), type IV (20%), and type I (6%). The percentage of SCC. mec type IV isolates was significantly higher in outpatients than in inpatients. Most of the isolated strains were sensitive to vancomycin (VCM, MIC ≤2 μg/mL), linezolid (MIC ≤4 μg/mL), and teicoplanin (MIC ≤8 μg/mL). Although most strains were sensitive to VCM, the MIC value of VCM for SCC. mec type II strains was higher than that for SCC. mec type IV strains. Only 4 (2.3%) of 171 SCC. mec type IV strains were Panton-Valentine leukocidin (lukS/F-PV)-positive. Thus, this result indicates a unique feature of SCC. mec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA.

AB - This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, including community-acquired MRSA (CA-MRSA), in Japan. A total of 857 MRSA clinical isolates were collected from the 16 institutions throughout Japan that participated in the survey (2008-2009). The drug susceptibility and staphylococcal cassette chromosome mec (SCC. mec) typing and the presence of specific pathogenic genes were evaluated. The isolates comprised SCC. mec type II (73.6%), type IV (20%), and type I (6%). The percentage of SCC. mec type IV isolates was significantly higher in outpatients than in inpatients. Most of the isolated strains were sensitive to vancomycin (VCM, MIC ≤2 μg/mL), linezolid (MIC ≤4 μg/mL), and teicoplanin (MIC ≤8 μg/mL). Although most strains were sensitive to VCM, the MIC value of VCM for SCC. mec type II strains was higher than that for SCC. mec type IV strains. Only 4 (2.3%) of 171 SCC. mec type IV strains were Panton-Valentine leukocidin (lukS/F-PV)-positive. Thus, this result indicates a unique feature of SCC. mec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA.

KW - Antimicrobial susceptibility

KW - CA-MRSA

KW - Molecular characteristic

KW - MRSA

KW - Nationwide survey

UR - http://www.scopus.com/inward/record.url?scp=84856608437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856608437&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2011.11.010

DO - 10.1016/j.diagmicrobio.2011.11.010

M3 - Article

C2 - 22244779

AN - SCOPUS:84856608437

VL - 72

SP - 253

EP - 257

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 3

ER -