Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009): Nationwide survey of community-acquired and nosocomial MRSA

Katsunori Yanagihara, Nobuko Araki, Shinichi Watanabe, Takahiro Kinebuchi, Mitsuo Kaku, Shigefumi Maesaki, Keizo Yamaguchi, Tetsuya Matsumoto, Hiroshige Mikamo, Yoshio Takesue, Jun Ichi Kadota, Jiro Fujita, Keiji Iwatsuki, Haruko Hino, Takehiko Kaneko, Kenji Asagoe, Masami Ikeda, Akira Yasuoka, Shigeru Kohno

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41 Citations (Scopus)

Abstract

This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, including community-acquired MRSA (CA-MRSA), in Japan. A total of 857 MRSA clinical isolates were collected from the 16 institutions throughout Japan that participated in the survey (2008-2009). The drug susceptibility and staphylococcal cassette chromosome mec (SCC. mec) typing and the presence of specific pathogenic genes were evaluated. The isolates comprised SCC. mec type II (73.6%), type IV (20%), and type I (6%). The percentage of SCC. mec type IV isolates was significantly higher in outpatients than in inpatients. Most of the isolated strains were sensitive to vancomycin (VCM, MIC ≤2 μg/mL), linezolid (MIC ≤4 μg/mL), and teicoplanin (MIC ≤8 μg/mL). Although most strains were sensitive to VCM, the MIC value of VCM for SCC. mec type II strains was higher than that for SCC. mec type IV strains. Only 4 (2.3%) of 171 SCC. mec type IV strains were Panton-Valentine leukocidin (lukS/F-PV)-positive. Thus, this result indicates a unique feature of SCC. mec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume72
Issue number3
DOIs
Publication statusPublished - Mar 2012

Keywords

  • Antimicrobial susceptibility
  • CA-MRSA
  • MRSA
  • Molecular characteristic
  • Nationwide survey

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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    Yanagihara, K., Araki, N., Watanabe, S., Kinebuchi, T., Kaku, M., Maesaki, S., Yamaguchi, K., Matsumoto, T., Mikamo, H., Takesue, Y., Kadota, J. I., Fujita, J., Iwatsuki, K., Hino, H., Kaneko, T., Asagoe, K., Ikeda, M., Yasuoka, A., & Kohno, S. (2012). Antimicrobial susceptibility and molecular characteristics of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009): Nationwide survey of community-acquired and nosocomial MRSA. Diagnostic Microbiology and Infectious Disease, 72(3), 253-257. https://doi.org/10.1016/j.diagmicrobio.2011.11.010